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Related Experiment Video

Updated: Jul 10, 2025

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Mineralized Microgels via Electrohydrodynamic Atomization: Optimization and In Vitro Model for Dentin-Pulp Complex.

Iriczalli Cruz-Maya1,2, Rosaria Altobelli1, Marco Antonio Alvarez-Perez2

  • 1Institute of Polymers, Composites and Biomaterials (IPCB), National Research Council of Italy, Mostra d'Oltremare Pad. 20, Viale J.F. Kennedy 54, 80125 Naples, Italy.

Gels (Basel, Switzerland)
|November 24, 2023
PubMed
Summary
This summary is machine-generated.

Bioactive composite microgels optimized via electrohydrodynamic atomization (EHDA) show promise for dentin-pulp complex regeneration. Mineralized microgels support in vitro osteogenesis and dental tissue regeneration.

Keywords:
atomizationcomposite hydrogelsdental tissue engineeringpulpar cells

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Dental Research

Background:

  • Micro-sized hydrogels offer advanced platforms for tissue regeneration by mimicking the cell niche.
  • Bioactive signals within hydrogels enhance their efficacy in regenerative applications.

Purpose of the Study:

  • To optimize bioactive composite microgels using electrohydrodynamic atomization (EHDA) for dentin-pulp complex regeneration.
  • To investigate the effect of mineral content on microgel properties and their interaction with dental pulp stem cells (DPSCs).

Main Methods:

  • Electrohydrodynamic atomization (EHDA) for microgel fabrication.
  • In situ mineral precipitation using disodium phosphate (Na2HPO4) and divalent ions.
  • Characterization using FTIR, TEM, EDAX, and image analysis.
  • In vitro studies with DPSCs on natural tooth slices.

Main Results:

  • EHDA produced uniformly rounded microgels (223-502 μm) with tunable mineral content.
  • Formation of calcium phosphates with a Ca/P ratio of ~1.67 and needle-like crystals confirmed.
  • Increased DPSC viability up to 14 days, followed by decreased proliferation suggesting differentiation.
  • Elevated ALP activity at 14 days indicated osteogenic potential.

Conclusions:

  • Mineralized microgels are effective platforms for supporting in vitro osteogenesis.
  • Optimized microgels show potential for dentin-pulp complex regeneration.
  • These microgels serve as valuable models for studying dental tissue regeneration.