Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

6.0K
Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such...
6.0K
SDS-PAGE01:27

SDS-PAGE

28.1K
Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact...
28.1K
DNA Agarose Gel Electrophoresis02:35

DNA Agarose Gel Electrophoresis

96.9K
Agarose gel electrophoresis is a laboratory technique commonly used to separate DNA fragments by size. However, it can also be used to isolate and purify DNA fragments using a gel extraction protocol.
Gel extraction follows five major steps: running gel electrophoresis to separate fragments, isolating the individual bands, extracting DNA from those bands, and removing the dye and salts from the extracted mixture to obtain pure DNA.
In cloning experiments, both the insert and vector DNA...
96.9K
Capillary Electrophoresis: Applications01:30

Capillary Electrophoresis: Applications

402
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
402

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phase behavior, self-assembly, and interfacial tension of a dynamically linked polymer blend.

The Journal of chemical physics·2026
Same author

Effects of Concentration, Salinity and Temperature on the Conformations of Zwitterionic Poly(2-Vinylpyridine‑<i>N</i>‑Oxide) Chains in Semidilute Solutions Probed by Small-Angle X‑Ray and Neutron Scattering.

Macromolecules·2026
Same author

Hierarchical relaxation and the microscopic origin of fast Li+ ions transport in Li7La3Zr2O12.

The Journal of chemical physics·2026
Same author

A Neutron Reflection Study of the Dissolution of Miscible Glassy Polymer Films over a Range of Temperature.

Macromolecules·2026
Same author

The Importance of Branch Placement on the Dilute Solution Properties of Comb-like Macromolecules.

Macromolecules·2026
Same author

Development of SAFT-based heteronuclear fused coarse-grained models of short alkanes and their mixtures with carbon dioxide and nitrogen.

The Journal of chemical physics·2025

Related Experiment Video

Updated: Jul 10, 2025

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

1.0K

Ion Partition in Polyelectrolyte Gels and Nanogels.

Alexandros Chremos1, Matan Mussel2, Jack F Douglas3

  • 1Section on Quantitative Imaging and Tissue Sciences, Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Gels (Basel, Switzerland)
|November 24, 2023
PubMed
Summary

This study models ion partitioning in polyelectrolyte gels, finding divalent ions partition more than monovalent ions. Neglecting solvation effects provides a simplified model but requires future refinement for accuracy.

Keywords:
gelsion partitionpolyelectrolyte gels

More Related Videos

Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE for Analysis of Multiprotein Complexes from Cellular Lysates
12:03

Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE for Analysis of Multiprotein Complexes from Cellular Lysates

Published on: February 24, 2011

131.4K
Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants
11:32

Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants

Published on: December 23, 2013

11.9K

Related Experiment Videos

Last Updated: Jul 10, 2025

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization
06:26

Synthesis of Stimuli-responsive Nanogels using Aqueous One-step Crosslinking and Co-nanopolymerization

Published on: January 24, 2025

1.0K
Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE for Analysis of Multiprotein Complexes from Cellular Lysates
12:03

Blue Native Polyacrylamide Gel Electrophoresis BN-PAGE for Analysis of Multiprotein Complexes from Cellular Lysates

Published on: February 24, 2011

131.4K
Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants
11:32

Characteristics of Precipitation-formed Polyethylene Glycol Microgels Are Controlled by Molecular Weight of Reactants

Published on: December 23, 2013

11.9K

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Physical Chemistry

Background:

  • Polyelectrolyte gels form structural frameworks in biological tissues and facilitate ion transport.
  • Understanding ion distribution within these gels is crucial for biological functions.

Purpose of the Study:

  • To model ion partitioning in polyelectrolyte gels with monovalent or divalent salts.
  • To compare ion distribution inside the gel with the bulk solution.
  • To establish a baseline model neglecting solvation effects.

Main Methods:

  • Developed an idealized polymer network model without explicit solvent description.
  • Investigated ion partitioning coefficients for monovalent and divalent ions.
  • Analyzed the influence of salt concentration and Bjerrum length (lB).

Main Results:

  • Ion partition coefficients scale with salt concentration.
  • Divalent ions show higher partition coefficients than monovalent ions across various Bjerrum lengths.
  • Divalent ions exhibit higher partitioning than monovalent ions at low concentrations and low lB when both are present.

Conclusions:

  • The simplified model provides a reference but neglects crucial solvation effects.
  • Accurate modeling of ion partitioning in polyelectrolyte gels requires incorporating ion and polymer solvation.
  • Further research should focus on including solvation for a more realistic description.