Electrosprayed Nanoparticles Containing Mangiferin-Rich Extract from Mango Leaves for Cosmeceutical Application

Area of Science:

• Pharmacology
• Nanotechnology
• Cosmeceuticals

Background:

• Mango (Mangifera indica L.) leaves contain mangiferin, a compound with significant antioxidant, anti-inflammatory, and antimicrobial properties.
• Mangiferin's potential therapeutic applications extend to various diseases, making it a valuable natural active compound.
• Traditional uses of mango highlight its biological activities, driving research into its pharmacological components.

Purpose of the Study:

• To prepare a mangiferin-rich extract (MRE) from mango leaves.
• To develop mangiferin-rich extract nanoparticles (MNPs) using an electrospraying technique for cosmeceutical applications.
• To investigate the cosmeceutical mechanisms of MRE and evaluate the efficacy of MNPs in transdermal delivery.

Main Methods:

• Preparation of mangiferin-rich extract (MRE) from mango leaves.
• Development of MRE-loaded nanoparticles (MNPs) using electrospraying with Eudragit® L100.
• Proteomic analysis to elucidate MRE's cosmeceutical mechanisms, including actin-filament organization and metalloenzyme-activity regulation.
• Characterization of MNPs (size, PDI) and quantification of encapsulation efficiency.
• Skin-retention study comparing MNP-containing emulsion-gel membranes with MRE solution.

Main Results:

• The electrospraying technique successfully produced MNPs with a mean size of 247.8 nm and a PDI of 0.271.
• High MRE entrapment efficiency of 84.9% was achieved, indicating effective encapsulation.
• Proteomic analysis revealed MRE's involvement in actin-filament organization and metalloenzyme-activity regulation, relevant to cosmeceutical effects.
• The skin-retention study demonstrated significantly higher mangiferin content in MNP-containing membranes compared to MRE solution membranes.

Conclusions:

• Electrospraying is an effective method for developing mangiferin-rich extract nanoparticles (MNPs) with high encapsulation efficiency.
• The developed MNPs show potential for enhanced transdermal delivery of mangiferin for cosmetic applications.
• Mangiferin-rich extract exhibits cosmeceutical benefits through mechanisms like actin-filament organization and metalloenzyme-activity regulation.