Subunit-exchange chromatography of self-associating proteins: a quantitative reappraisal

A quantitative expression describing the behavior of a self-associating protein in subunit-exchange chromatography is derived in a form that is tractable from the viewpoint of characterizing the pertinent interactions. Its use is illustrated by application to published results for alpha-chymotrypsin, oxyhemoglobin, and the light-harvesting chlorophyll a/b protein.