Related Experiment Video
Updated: Jul 10, 2025
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G Protein-selective GPCR Conformations Measured Using FRET Sensors in a Live Cell Suspension Fluorometer Assay
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GproteinDb in 2024: new G protein-GPCR couplings, AlphaFold2-multimer models and interface interactions
Gáspár Pándy-Szekeres1,2, Luis P Taracena Herrera1, Jimmy Caroli1
1Department of Drug Design and Pharmacology, University of Copenhagen, 2100 Copenhagen, Denmark.
Nucleic Acids Research
|November 24, 2023
Summary
G protein-coupled receptors (GPCRs) are crucial for drug action. GproteinDb now offers expanded data and advanced tools, including AlphaFold2 models, to analyze GPCR-G protein interactions and selectivity.
Area of Science:
- Biochemistry
- Pharmacology
- Structural Biology
Background:
- G proteins are key signaling molecules interacting with approximately 800 types of receptors.
- These receptors bind to various ligands, including medicines, hormones, neurotransmitters, tastants, and odorants.
- GproteinDb serves as a resource for genomic, structural, and pharmacological data related to these systems.
Purpose of the Study:
- To present a major update to the GproteinDb database and analysis tools.
- To expand the available data on G protein coupling and structural templates.
- To incorporate novel structural models and enhance interactive analysis of GPCR-G protein interfaces.
Main Methods:
- Integration of expanded G protein coupling data and structural templates.
- Addition of AlphaFold2-predicted structure models for GPCR-G protein complexes.
- Advancement of interactive tools for analyzing GPCR-G protein interface selectivity.
- Analysis of coupling agreement across different datasets and parameters (constitutive activity, agonist-induced activity, kinetics).
Main Results:
- GproteinDb has been significantly updated with expanded coupling data and structural templates.
- Novel AlphaFold2 models of GPCR-G protein complexes are now available.
- Enhanced interactive tools facilitate the analysis of coupling selectivity at the interface.
- Insights into coupling agreement across various datasets and activity parameters have been generated.
Conclusions:
- The updated GproteinDb provides a comprehensive resource for studying GPCR-G protein interactions.
- The inclusion of AlphaFold2 models and advanced tools aids in understanding coupling selectivity.
- The database supports experiment design and analysis in pharmacology and structural biology.
- GproteinDb is a valuable, accessible platform for researchers in the field.
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