High specificity THz metamaterial-based biosensor for label-free transcription factor detection in melanoma...

Area of Science:

• Biomedical Engineering
• Metamaterials Science
• Oncology Diagnostics

Background:

• Melanoma diagnosis relies on accurate detection of specific biomarkers.
• Transcription factors, like early growth response protein 2 (EGR2), are crucial in melanoma cell activity.
• Current diagnostic methods may lack the sensitivity for early-stage detection.

Purpose of the Study:

• To introduce a novel terahertz biosensor for the selective and sensitive detection of EGR2.
• To demonstrate the potential of this biosensor as a diagnostic tool for melanoma.
• To investigate the application of frequency selective surface metamaterials in biological sensing.

Main Methods:

• Design and fabrication of a terahertz biosensor using a 2D array of asymmetrically, doubly split ring resonator unit cells.
• Utilizing slits in a metallic layer with an undercut etch for selective functionalization.
• Testing the sensor's ability to detect early growth response protein 2 (EGR2) in complex cellular protein samples.

Main Results:

• The terahertz biosensor demonstrated selective and sensitive detection of EGR2.
• The sensor design allows for increased sensitivity through functionalization of the active area.
• Feasible specific detection of the target transcription factor was achieved.

Conclusions:

• The developed terahertz biosensor shows significant promise as a diagnostic tool for melanoma.
• The sensor's ability to detect EGR2 offers a new avenue for early melanoma detection.
• Metamaterial-based biosensors represent a powerful platform for sensitive biomarker detection in complex biological samples.