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Dual-color Correlative Light and Electron Microscopy for the Visualization of Interactions between Mitochondria and Lysosomes
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Published on: September 27, 2024

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Lycopene Maintains Mitochondrial Homeostasis to Counteract the Enterotoxicity of Deoxynivalenol

Zihui Cai1, Fengjuan Chen1, Youshuang Wang1

  • 1College of Veterinary Medicine, Henan Agricultural University, No.15 Longzihu University Park, Zhengdong New District, Zhengzhou 450046, China.

Antioxidants (Basel, Switzerland)
|November 25, 2023
PubMed

Insights

Lycopene (LYC) protects intestinal cells from Deoxynivalenol (DON) toxicity by maintaining mitochondrial homeostasis. LYC reduces oxidative stress and restores mitochondrial function damaged by DON exposure.

Area of Science:

  • Cell Biology
  • Toxicology
  • Nutritional Science

Background:

  • The intestinal tract is susceptible to Deoxynivalenol (DON) toxicity.
  • Mitochondrial homeostasis imbalance is a key mechanism in DON-induced gut toxicity.
  • Lycopene (LYC) exhibits intestinal protective properties and can maintain mitochondrial homeostasis.

Purpose of the Study:

  • To investigate the protective effects of Lycopene (LYC) against Deoxynivalenol (DON)-induced damage in intestinal porcine epithelial cells (IPEC-J2).

Main Methods:

  • DON exposure was used to induce oxidative stress and mitochondrial dysfunction in IPEC-J2 cells.
  • Measurements included malondialdehyde and reactive oxygen species (ROS) levels.
  • Assessed effects on cell barrier function, mitochondrial permeability transition pore (MPTP) opening, mitochondrial membrane potential (MMP), and expression of mitochondrial dynamics and mitophagy factors.

Main Results:

  • DON exposure increased malondialdehyde and ROS levels, impaired cell barrier function, and caused mitochondrial dysfunction in IPEC-J2 cells.
  • DON disrupted mitochondrial dynamics and mitophagy processes.
  • Lycopene supplementation mitigated DON-induced cellular oxidative stress, functional damage, and mitochondrial imbalance.

Conclusions:

  • Lycopene (LYC) effectively counteracts Deoxynivalenol (DON) toxicity in intestinal cells.
  • LYC maintains mitochondrial homeostasis, thereby protecting against DON-induced gut damage.
Keywords:
DeoxynivalenolIPEC-J2 cellsLycopenemitochondrial homeostasis

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