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Related Concept Videos

What is Cancer?02:12

What is Cancer?

Cells and tissues must meticulously coordinate their activities for the normal functioning of the human body. Therefore, they exhibit socially responsible behavior - resting, growing, dividing, differentiating, or dying - for the organism’s benefit. Cancer arises when cells divide uncontrollably and invade other tissues or organs.
Although people have known about cancer for centuries, it was only in 1761 that Giovanni Morgagni of Padua performed a detailed autopsy of patients who died from...
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...
Adaptive Mechanisms in Cancer Cells02:53

Adaptive Mechanisms in Cancer Cells

Cancer cells accumulate genetic changes at an abnormally rapid rate due to the defects in the DNA repair mechanisms. From an evolutionary perspective, such genetic instability is advantageous for cancer development. Mutant cell lines accumulate a series of beneficial mutations that contribute to their progression into cancer.
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...

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ZW4864-mediated inhibition of the β-catenin/BCL9/BCL9L complex reveals therapeutic potential in bladder cancer.

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Ionizing radiation and photodynamic therapy lead to multimodal tumor cell death, synergistic cytotoxicity and immune cell invasion in human bladder cancer organoids.

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Updated: Jun 30, 2026

Culture, Manipulation, and Orthotopic Transplantation of Mouse Bladder Tumor Organoids
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Cell Line-Based Human Bladder Organoids with Bladder-like Self-Organization-A New Standardized Approach in Bladder

Mandy Berndt-Paetz1, Shanfu Han2, Annett Weimann1

  • 1Department of Urology, Research Laboratories, Leipzig University, 04103 Leipzig, Germany.

Biomedicines
|November 25, 2023
PubMed
Summary
This summary is machine-generated.

Bladder cancer organoids, mimicking tumor structure and drug responses, offer valuable models for research. These three-dimensional models aid in understanding bladder cancer and developing new anti-cancer therapies.

Keywords:
Wnt/β-catenin activationbladder cancer cell linesdrug responseorganoidsself-organizationstratification

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Area of Science:

  • Oncology
  • Biotechnology
  • Cancer Research

Background:

  • Three-dimensional (3D) organoids are crucial for advancing bladder cancer (BCa) research.
  • Organoids composed of multiple cell types accurately replicate solid tumors' architecture, proliferation, cell interactions, and drug responses.

Purpose of the Study:

  • To develop and characterize novel 3D organoid models from human BCa cell lines and bladder stromal cells.
  • To assess the utility of these organoids for studying BCa biology and evaluating anti-cancer therapies.

Main Methods:

  • Development of four organoids using human BCa cell lines with bladder fibroblasts and smooth muscle cells.
  • Characterization via immunoreactivity for cytokeratins, vimentin, α-actin, and KI67.
  • Investigation of Wnt/β-catenin pathway activation and response to doxorubicin and radiotherapy.

Main Results:

  • Organoids exhibited a bladder wall-like structure with urothelial cells and a supportive cell core.
  • Immunoreactivity and proliferation rates correlated with BCa cell origins; RT-112 organoids showed epithelial stratification.
  • Wnt10B treatment activated β-catenin in high-grade organoids. Doxorubicin reduced viability by 10-30%. Radiotherapy efficacy varied with proliferation status.

Conclusions:

  • Cell-line-based organoids successfully recapitulate bladder-like structures and in vivo features, including urothelial differentiation.
  • These organoids serve as effective tools for functional studies in bladder cancer research.
  • The developed organoids show promise for anti-cancer drug development and personalized medicine approaches.