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The Analysis of Selected miRNAs and Target MDM2 Gene Expression in Oral Squamous Cell Carcinoma
Karolina Gołąbek1, Dorota Hudy1, Jadwiga Gaździcka1
1Department of Medical and Molecular Biology, Faculty of Medical Sciences in Zabrze, Medical University of Silesia in Katowice, 19 Jordana St., 41-808 Zabrze, Poland.
Abstract:
MiRNAs could play an important role in tumorigenesis and progression. The oncoprotein MDM2 (murine double minute 2) was identified as a negative regulator of the tumour suppressor p53. This study aims to analyse the expression of the MDM2 target miRNA candidates (miR-3613-3p, miR-371b-5p and miR-3658) and the MDM2 gene in oral squamous cell carcinoma tumour and margin samples and their association with the selected socio-demographic and clinicopathological characteristics. The study group consisted of 50 patients. The miRNAs and MDM2 gene expression levels were assessed by qPCR. The expression analysis of the miRNAs showed the expression of only one of them, i.e., miR-3613-3p. We found no statistically significant differences in the miR-3613-3p expression in tumour samples compared to the margin samples. When analysing the effect of smoking on miR-3613-3p expression, we demonstrated a statistically significant difference between smokers and non-smokers. In addition, we showed an association between the miR-3613-3p expression level and some clinical parameters in tumour samples (T, N and G). Our study demonstrates that miR-3613-3p overexpression is involved in the tumour progression of OSCC. This indicates that miR-3613-3p possesses potential prognostic values.
Insights
This study investigated microRNA-3613-3p (miR-3613-3p) in oral squamous cell carcinoma (OSCC). Overexpression of miR-3613-3p is linked to tumor progression and may serve as a prognostic marker in OSCC patients.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- MicroRNAs (miRNAs) are implicated in cancer development and progression.
- Murine double minute 2 (MDM2) is an oncoprotein that negatively regulates the tumor suppressor p53.
- MDM2 targets specific miRNAs, including potential candidates like miR-3613-3p, miR-371b-5p, and miR-3658.
Purpose of the Study:
- To analyze the expression of MDM2 target miRNAs (miR-3613-3p, miR-371b-5p, miR-3658) and the MDM2 gene in oral squamous cell carcinoma (OSCC) and adjacent margin tissues.
- To investigate the association of these miRNA and gene expressions with socio-demographic and clinicopathological characteristics in OSCC patients.
Main Methods:
- Quantitative real-time PCR (qPCR) was used to assess miRNA and MDM2 gene expression levels.
- The study included 50 OSCC patients, analyzing both tumor and margin samples.
Main Results:
- Only miR-3613-3p was detected among the targeted miRNAs.
- No significant difference in miR-3613-3p expression was observed between tumor and margin samples.
- A statistically significant difference in miR-3613-3p expression was found between smokers and non-smokers.
- miR-3613-3p expression levels were associated with clinical parameters such as tumor size (T), lymph node involvement (N), and tumor grade (G).
Conclusions:
- Overexpression of miR-3613-3p is involved in the tumor progression of oral squamous cell carcinoma (OSCC).
- miR-3613-3p demonstrates potential as a prognostic biomarker for OSCC.
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