Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prolotherapy versus epidural steroid injections for lumbar pain with associated leg pain: A pragmatic randomized controlled trial.

PM & R : the journal of injury, function, and rehabilitation·2026
Same author

Novel Sonoguided Digital Palpation and Hydrodissection for Sural Nerve Dysfunction Mimicking Achilles Tendinopathy in a Psoriasis Patient.

Diagnostics (Basel, Switzerland)·2025
Same author

Ultrasonographic Diagnosis and Computed Tomographic Confirmation of a Scapular Body Stress Fracture in an Elite Boxer: A Case Report.

Diagnostics (Basel, Switzerland)·2025
Same author

Innovative Dynamic Ultrasound Diagnosis of First Rib Stress Fracture in an Adolescent Athlete-A Case Report.

Diagnostics (Basel, Switzerland)·2025
Same author

Ultrasound-Guided Prolotherapy for Sciatica Secondary to Sacrospinous Ligament Calcification: A Potential and Previously Overlooked Etiological Factor in Deep Gluteal Syndrome-A Case Report and Literature Review.

Life (Basel, Switzerland)·2025
Same author

Ultrasound-Guided Hydrodissection with Needle Stabilization: An Innovative Nerve-Sparing Approach to Remove a Contraceptive Implant Causing Ulnar Neuropathy.

Diagnostics (Basel, Switzerland)·2025
Same journal

Correction: Komatsu et al. Three-Dimensional Visualization and Detection of the Pulmonary Venous-Left Atrium Connection Using Artificial Intelligence in Fetal Cardiac Ultrasound Screening. <i>Bioengineering</i> 2026, <i>13</i>, 100.

Bioengineering (Basel, Switzerland)·2026
Same journal

Comparison of CO<sub>2</sub> Laser and Microdebrider in the Surgical Treatment of Pediatric Recurrent Respiratory Papillomatosis: A Retrospective Analysis.

Bioengineering (Basel, Switzerland)·2026
Same journal

Toward More Translational Tumor Models: Breast dECM-Based 3D Systems Capture Native Microenvironmental Cues.

Bioengineering (Basel, Switzerland)·2026
Same journal

Postural Stability Changes During the 4 Phases of the Half Squat: Kinematics Profile of the Center of Pressure and Center of Mass in High-Performance Weightlifters-A Pilot Study.

Bioengineering (Basel, Switzerland)·2026
Same journal

Definite Implant Position as Novel Readout for Effectiveness of Ridge Preservation Indicates to Beneficial Effect of Combined Treatment with Platelet-Rich Fibrin (PRF) and Xenogenic Biomaterial in Bone Regeneration.

Bioengineering (Basel, Switzerland)·2026
Same journal

Trueness and Precision of Intraoral Scanners for 3D-Printed Orthodontic Models with Attachments: An In Vitro Comparative Study.

Bioengineering (Basel, Switzerland)·2026
See all related articles

Related Experiment Video

Updated: Jul 10, 2025

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis
14:38

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Published on: January 4, 2019

18.3K

Optimizing Platelet-Rich Plasma: Spin Time and Sample Source.

Theodore E Harrison1, Jannice Bowler2, Chin-I Cheng3

  • 1Independent Researcher, Victoria, BC V8L 5K1, Canada.

Bioengineering (Basel, Switzerland)
|November 25, 2023
PubMed
Summary
This summary is machine-generated.

Platelets (PLTs) are not solely in the buffy coat after blood centrifugation. Optimal platelet recovery involves aspirating a 1 mL volume centered around the buffy coat layer.

Keywords:
buffy coatplatelet-rich plasmaplatelets

More Related Videos

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

581
Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
07:13

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Published on: January 7, 2019

8.0K

Related Experiment Videos

Last Updated: Jul 10, 2025

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis
14:38

Preparation, Procedures and Evaluation of Platelet-Rich Plasma Injection in the Treatment of Knee Osteoarthritis

Published on: January 4, 2019

18.3K
Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets
05:49

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

581
Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
07:13

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Published on: January 7, 2019

8.0K

Area of Science:

  • Hematology
  • Biomedical Engineering

Background:

  • The buffy coat, a layer in centrifuged blood, traditionally separates plasma from red blood cells (RBCs).
  • The distribution of platelets (PLTs) and leukocytes (WBCs) within centrifuged blood layers is not well-characterized.

Purpose of the Study:

  • To describe the distribution of platelets and leukocytes in centrifuged blood.
  • To determine optimal methods for platelet-rich plasma or buffy coat layer aspiration.

Main Methods:

  • Centrifugation of anticoagulated blood samples (8.5 mL) at 1000× g for varying durations (1-20 min).
  • Division of centrifuged samples into ten layers for cellular composition analysis.
  • Analysis of cellular composition and mean platelet volume (MPV) in each layer.

Main Results:

  • Significant percentages of platelets (15% in plasma, 65% in RBCs) remain outside the buffy coat even after 20 min of centrifugation.
  • Optimal platelet recovery is achieved by aspirating a 1 mL volume centered around the buffy coat.
  • Maximum platelet counts are obtained within 5 min of centrifugation using this aspiration method.

Conclusions:

  • Standard buffy coat aspiration methods may lead to suboptimal platelet recovery.
  • A revised aspiration technique, encompassing a broader layer around the buffy coat, enhances platelet yield.
  • Understanding platelet distribution is crucial for optimizing blood component preparation.