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Related Experiment Videos

Disulfiram as a radiation modifier.

R D Taylor, A W Maners, H Salari

    Annals of Clinical and Laboratory Science
    |November 1, 1986
    PubMed
    Summary
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    Tetraethylthiuram disulfide (disulfiram) did not show radiation modifying effects in L-929 cells at a concentration of 0.1 microgram/ml. Further research at different concentrations may reveal potential radioprotective properties due to its structural similarity to DTC.

    Area of Science:

    • Biochemistry
    • Radiology
    • Pharmacology

    Background:

    • Tetraethylthiuram disulfide (disulfiram, DSM) is known to inhibit key enzymes like aldehyde dehydrogenase and cytochrome P-450.
    • Disulfiram is utilized in alcoholism aversion therapy and has demonstrated inhibition of carcinogen-induced tumor formation.
    • Disulfiram exhibits a biphasic toxicity pattern in L-929 mouse fibroblast cultures, similar to its metabolite, sodium diethyldithiocarbamate (DTC).

    Purpose of the Study:

    • To investigate the radiation modifying effects of tetraethylthiuram disulfide (disulfiram).
    • To assess the toxicity and potential radioprotective or radiosensitizing properties of disulfiram.
    • To compare the radiation modifying potential of disulfiram with its metabolite, sodium diethyldithiocarbamate (DTC).

    Main Methods:

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    • Utilized the L-929 mouse fibroblast cell culture system.
    • Assessed the toxicity of disulfiram across a range of concentrations, identifying a biphasic toxicity pattern.
    • Evaluated the effect of disulfiram at 3 X 10(-7) M (0.1 microgram/ml) on the survival of irradiated cells.

    Main Results:

    • Disulfiram demonstrated a biphasic toxicity pattern in L-929 cells, with 100% toxicity at 2 X 10(-7) M and 3.4 X 10(-6) M, and 23% toxicity at 3 X 10(-7) M.
    • Disulfiram at 0.1 microgram/ml did not significantly alter the survival of irradiated L-929 cells compared to the control group.
    • No significant difference in the Do (cell survival curve shoulder) was observed between irradiated cells treated with disulfiram and the irradiated control group.

    Conclusions:

    • Tetraethylthiuram disulfide (disulfiram) did not exhibit significant radiation modifying effects at the tested concentration of 0.1 microgram/ml in L-929 cells.
    • The observed biphasic toxicity of disulfiram mirrors that of its metabolite, sodium diethyldithiocarbamate (DTC).
    • Given the structural similarity to DTC, which has shown radioprotective effects, disulfiram might possess radioprotective properties at different concentrations.