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Related Experiment Video

Updated: Jul 10, 2025

Establishment of a Novel Ex Vivo Lung Perfusion System for Rat Lungs After Circulatory Death
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Establishment of a Novel Ex Vivo Lung Perfusion System for Rat Lungs After Circulatory Death

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Post-Mortem Extracorporeal Membrane Oxygenation Perfusion Rat Model: A Feasibility Study.

Matthias Manfred Deininger1,2, Carl-Friedrich Benner3, Lasse Johannes Strudthoff4

  • 1Department of Intensive and Intermediate Care, Medical Faculty, RWTH Aachen University, 52074 Aachen, Germany.

Animals : an Open Access Journal From MDPI
|November 25, 2023
PubMed
Summary

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Evaluating a Novel Cell-Free Preservation Solution for Human Cardiomyocyte Protection: A Proof-of-Concept Study.

BioMed research international·2026
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Association between sustained moderate hyperglycemia within first 48 hours and poor functional outcome after polytrauma: A retrospective cohort study.

European journal of trauma and emergency surgery : official publication of the European Trauma Society·2026
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Real-Time Subject-Specific Predictive Modeling of PPG Signals for Artifact-Resilient SpO2 Estimation Under Hypoxia.

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Computer-Interpretable Quality Indicators for Intensive Care Medicine: Development and Validation Study.

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Efficacy and Safety of a Balanced Gelatine Solution for Fluid Resuscitation in Sepsis: A Prospective, Randomised, Controlled, Double-Blind Trial-GENIUS Trial.

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Assembling, Priming, and Storing Circuits for Pediatric and Neonatal Extracorporeal Membrane Oxygenation.

ASAIO journal (American Society for Artificial Internal Organs : 1992)·2025

This study introduces a novel post-mortem rat perfusion model using extracorporeal membrane oxygenation (ECMO) in surplus cadavers. This approach offers a viable alternative for biomedical device testing, aligning with the 3Rs principle by reducing live animal use.

Area of Science:

  • Biomedical Engineering
  • Physiology
  • Animal Models

Background:

  • Biomedical device development necessitates animal testing, prompting efforts to reduce live animal use per the 3Rs principle.
  • A significant number of surplus animals are euthanized without scientific utilization, presenting an opportunity for alternative research models.

Purpose of the Study:

  • To establish a post-mortem rat perfusion model utilizing extracorporeal membrane oxygenation (ECMO) in surplus rat cadavers.
  • To evaluate the oxygenation performance of a novel ECMO membrane oxygenator in this post-mortem model.

Main Methods:

  • Four euthanized rats were connected to a venous-arterial ECMO system for up to eight hours.
  • Organ perfusion was assessed using angiographic imaging and hemodynamic monitoring (mean femoral arterial pressure).
Keywords:
3RsECMOSeldinger techniqueoxygenatorperfusionpost vivopost-mortemrat modelsurplus

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  • Blood gas analyses and oxygenation calculations were performed to evaluate oxygen delivery and consumption.

    • Main Results:

    • The ECMO system preparation time averaged 791 ± 29 seconds.
    • Sufficient organ perfusion was maintained for an average of 463 ± 26 minutes.
    • A 73% increase in arterial oxygen concentration and a stable partial oxygen pressure were observed, with an oxygen extraction ratio increasing up to 4.75 times.

    Conclusions:

    • The established post-mortem ECMO perfusion rat model using surplus animals is a promising alternative to live animal models, adhering to the 3Rs.
    • The model's ability to maintain organ perfusion and oxygenation makes it suitable for various biomedical device testing applications.