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Macrocystis pyrifera Lipids Reduce Cytokine-Induced Pro-Inflammatory Signalling and Barrier Dysfunction in Human
Jamie M L Kok1, Georgina C Dowd2, Jaydee D Cabral3
1Department of Pharmacology and Toxicology, School of Biomedical Sciences, University of Otago, Dunedin 9054, New Zealand.
International Journal of Molecular Sciences
|November 25, 2023
Summary
Lipids from Macrocystis pyrifera seaweed reduce inflammation and protect the skin barrier. These findings suggest potential benefits for treating atopic dermatitis and other skin conditions.
Area of Science:
- Dermatology
- Marine Biotechnology
- Immunology
Background:
- Atopic dermatitis involves epidermal barrier dysfunction and inflammation.
- Macrocystis pyrifera lipids may possess anti-inflammatory properties.
Purpose of the Study:
- To investigate the effects of Macrocystis pyrifera lipids on keratinocytes.
- To assess the barrier-protective and anti-inflammatory potential of these lipids.
Main Methods:
- Treatment of HaCaT keratinocytes with Macrocystis pyrifera lipids.
- Stimulation with tumor necrosis factor-alpha (TNF-α), interleukin-4 (IL-4), and interleukin-13 (IL-13).
- Analysis of tight junction proteins, chemoattractant protein production, and trans-epidermal water loss in 2D and 3D cell cultures.
Main Results:
- Macrocystis pyrifera lipids inhibited TNF-α-induced production of TNF receptor-associated factor 2 and monocyte chemoattractant protein-1 (MCP-1).
- Lipid pre-treatment reduced MCP-1 production, claudin-1 tight junction loss, and trans-epidermal leakage in 3D keratinocyte cultures.
- Limited improvement in tight junction integrity was observed in 2D cultures.
Conclusions:
- Macrocystis pyrifera lipids demonstrate anti-inflammatory effects on keratinocytes.
- These lipids exhibit barrier-protective properties, particularly in a 3D skin model.
- Macrocystis pyrifera lipids show promise for managing atopic dermatitis and related skin disorders.
