Volumetric Temperature Mapping Using Light-Sheet Microscopy and Upconversion Fluorescence from Micro- and Nano-Rare...

Area of Science:

• Materials Science
• Nanotechnology
• Optical Physics

Background:

• Accurate temperature mapping is crucial for understanding material properties and processes.
• Upconversion nanoparticles (UCNPs) offer unique optical properties for sensing applications.
• Existing 3D temperature mapping techniques can be complex and limited in scale.

Purpose of the Study:

• To present a simple and effective 3D temperature mapping technique.
• To demonstrate the feasibility of the technique using rare-earth doped nanoparticles.
• To enable precise temperature measurements in various host materials.

Main Methods:

• Combining light-sheet excitation with two-dimensional fluorescence intensity ratio (FIR) measurements.
• Utilizing sodium yttrium fluoride nanoparticles (NaYF4:Yb3+/Er3+) as temperature probes.
• Employing a single CMOS camera and interferometric filters to capture specific fluorescence bands (525 nm and 550 nm).

Main Results:

• Successfully demonstrated 3D temperature mapping in polydimethylsiloxane (PDMS) and agar-based samples.
• Achieved optically sectioned images with good resolution over millimetric scales.
• Obtained 2D FIR maps directly correlating to temperature variations.

Conclusions:

• The developed light-sheet FIR technique is a simple and promising method for 3D temperature mapping.
• The technique is effective with both bulk composite materials and nanoparticle suspensions.
• This approach facilitates 3D temperature reconstruction for various scientific and technological applications.