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Organic-Solvent-Resistant Polyimide/Hydroxyapatite Mixed Matrix Membranes for Lysozyme Adsorption
Junfen Sun1, Hao Pang1, Long Chen1
1State Key Laboratory for Modification of Chemical Fibers and Polymer Materials, College of Material Science and Engineering, Donghua University, North People Road 2999, Shanghai 201620, China.
Materials (Basel, Switzerland)
|November 25, 2023
Summary
New polyimide/hydroxyapatite (PI/HAP) mixed matrix membranes (MMMs) effectively adsorb enzymes from organic solvents. These crosslinked MMMs show high adsorption capacity and recyclability, offering a novel purification technology.
Area of Science:
- Materials Science
- Biotechnology
- Chemical Engineering
Background:
- Enzyme purification from organic solvents presents challenges.
- Mixed matrix membranes (MMMs) offer potential for selective adsorption.
- Polyimide/hydroxyapatite (PI/HAP) composites are explored for membrane applications.
Purpose of the Study:
- To develop and characterize novel polyimide/hydroxyapatite (PI/HAP) mixed matrix membranes (MMMs).
- To investigate the crosslinking effects of 3-aminopropyl triethoxysilane (APTES) on MMM stability and performance.
- To evaluate the adsorption capacity and recyclability of the developed MMMs for lysozyme (LZ) in organic solvents.
Main Methods:
- Preparation of PI/HAP MMMs using the phase inversion method.
- Crosslinking of MMMs with varying concentrations and times of APTES.
- Characterization of chemical and structural stability of the crosslinked MMMs.
- Lysozyme (LZ) adsorption studies in different organic solvents.
Main Results:
- Optimal crosslinking achieved with 6% APTES for 8 hours, enhancing stability.
- PI/HAP MMMs demonstrated high LZ adsorption capacity (34.1 mg/g) in organic-solvent-aqueous solutions.
- The MMMs exhibited significant desorption capacity and recyclability, retaining 60% capacity after four cycles.
Conclusions:
- Crosslinked PI/HAP MMMs provide an effective platform for enzyme adsorption in organic media.
- The developed MMMs offer a promising technology for enzyme and protein purification and separation.
- This study highlights the potential of tailored MMMs for bioseparation processes.
