Stereoselective Voltammetric Biosensor for Myo-Inositol and D-Chiro-Inositol Recognition

Area of Science:

• Electrochemistry
• Biosensor Development
• Chiral Analysis

Background:

• Stereoselective discrimination of inositol stereoisomers is crucial for pharmaceutical quality control.
• Existing methods for chiral analysis can be complex and time-consuming.
• Development of rapid and accurate biosensing platforms is needed.

Purpose of the Study:

• To develop a simple and stereoselective voltammetric biosensor for differentiating myo-inositol and D-chiro-inositol.
• To characterize the biosensor's performance and evaluate its potential for pharmaceutical analysis.
• To investigate the interactions between bovine serum albumin and inositol stereoisomers on a modified electrode surface.

Main Methods:

• Functionalization of a multi-walled carbon nanotube graphite screen-printed electrode (MWCNT-GSPE) with methylene blue (MB).
• Adsorption of bovine serum albumin (BSA) onto the functionalized electrode to create the biosensing platform.
• Electrochemical characterization using differential pulse voltammetry (DPV) and electrochemical impedance spectroscopy (EIS).

Main Results:

• The developed biosensor demonstrated stereoselective discrimination between myo-inositol and D-chiro-inositol.
• Higher binding affinity was observed between myo-inositol and BSA compared to D-chiro-inositol and BSA.
• Linear response in the range of 2-100 μM with low limits of detection (LODs) for both stereoisomers.
• Successful determination of stereoisomeric composition in commercial pharmaceutical preparations.

Conclusions:

• The BSA/MB/MWCNT/GSPE platform offers a promising approach for the stereoselective analysis of inositol isomers.
• The biosensor exhibits potential for application in chiral analysis of pharmaceuticals and forensic samples.
• This method provides a simple, efficient, and accurate tool for distinguishing between myo-inositol and D-chiro-inositol.