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Characterization of Two Aggressive PepMV Isolates Useful in Breeding Programs.

Cristina Alcaide¹, Eduardo Méndez-López¹, Jesús R Úbeda¹

¹"Del Segura" Centre for Applied Biology (CEBAS), Consejo Superior de Investigaciones Científicas (CSIC), 30100 Murcia, Spain.

|November 25, 2023

Summary

Aggressive Pepino mosaic virus (PepMV) variants, PepMV-H30 and PepMV-KLP2, were characterized. A specific substitution in the coat protein of PepMV-H30 was found to induce severe symptoms, offering tools for tomato resistance breeding.

Keywords:

PepMV strains bright yellow mosaic coat protein mutation symptom induction virus evolution

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Area of Science:

Plant Pathology
Virology
Genetics

Background:

Pepino mosaic virus (PepMV) causes significant economic losses in global tomato production.
Emergence of aggressive PepMV variants necessitates characterization for disease management.

Purpose of the Study:

To characterize two aggressive PepMV isolates, PepMV-H30 and PepMV-KLP2, from Spain.
To identify potential viral determinants responsible for severe symptom induction in tomato.

Main Methods:

Generation of full-length infectious clones for PepMV-H30 and PepMV-KLP2.
Phylogenetic analysis using nucleotide sequences of isolates.
Amino acid sequence comparison and site-directed mutagenesis (E236K substitution).

Main Results:

PepMV-H30 and PepMV-KLP2 were identified as EU and CH2 strains, respectively.
8 and 15 amino acid substitutions were identified in PepMV-H30 and PepMV-KLP2, respectively, compared to mild isolates.
The E236K substitution in the coat protein (CP) of PepMV-H30 induced severe symptoms in *Nicotiana benthamiana*.

Conclusions:

The E236K substitution in the PepMV CP is a potential determinant of severe symptom induction.
PepMV-H30 and PepMV-KLP2 are valuable resources for developing PepMV resistance in tomato breeding programs.