Related Experiment Video
Updated: Jul 10, 2025

Enhancing Efficiency and Radiolabeling Yields of Carbon-11 Radioligands for Clinical Research Using the Loop Method
Published on: December 20, 2024
11C-Fixation Techniques
Emily Murrell1, Anton Lindberg1, Armando Garcia1
1Azrieli Centre for Neuro-Radiochemistry, Brain Health Imaging Centre, Centre for Addiction and Mental Health, Toronto, ON, Canada.
Abstract:
This protocol describes the application of cyclotron-generated [11C]CO2 fixation reactions for direct 11C-carboxylation reactions and [11C]CO for 11C-carbonylations. Herein we describe one-pot methods wherein the radioactive gas is first trapped in a reaction mixture at room temperature and atmospheric pressure prior to the radiolabeling reactions. Such procedures are widely applicable to numerous small molecules to form 11C-labeled carboxylic acids, amides, esters, ketones, oxazolidinones, carbamates, and ureas. The steps for 11C-fixation techniques described herein are tailored for a commercial automated synthesis unit and are readily adapted for routine radiopharmaceutical production.
More Related Videos
08:05Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
Published on: October 7, 2020
07:26Metabolic Pathway Confirmation and Discovery Through 13C-labeling of Proteinogenic Amino Acids
Published on: January 26, 2012
Related Concept Videos
C4 Pathway and CAM
C4 Pathway
The C4 pathway is used by plants such as...
The Calvin Benson Cycle
The Calvin Cycle
Overview of Nitrogen Metabolism
The largest pool of nitrogen available in the terrestrial ecosystem is gaseous nitrogen (N2) from the air, but this...
Double Resonance Techniques: Overview
Spin decoupling is usually achieved by...
¹³C NMR: ¹H–¹³C Decoupling
A broadband decoupling technique is used to simplify these complex, sometimes overlapping, signals. Broadband decoupling relies on a...