Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Imaging Studies II: Positron Emission Tomography and Scintigraphy

Positron Emission Tomography (PET) is a medical imaging technique that provides crucial insights into the body's physiological functions at a molecular level. It is an indispensable resource for diagnosing, staging, and monitoring various illnesses, notably cancer, neurological disorders, and cardiovascular conditions.
Fundamental Principles of PET

Positron Emission Tomography

Positron Emission Tomography

Positron emission tomography (PET) is a medical imaging technique involving radiopharmaceuticals — substances that emit short-lived radiation. Although the first PET scanner was introduced in 1961, it took 15 more years before radiopharmaceuticals were combined with the technique and revolutionized its potential.
One of the main requirements of a PET scan is a positron-emitting radioisotope, which is produced in a cyclotron and then attached to a substance used by the part of the body...

Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

PD-L1 Immuno-PET Reveals Systemic Effects of Localized Oncolytic Virotherapy in a Mouse Model of Head and Neck Cancer.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine·2026

Same author

Fully Automated [<sup>18</sup>F]AlF Radiolabeling Using a Microdroplet Reactor Platform.

Chemical engineering journal (Lausanne, Switzerland : 1996)·2026

Same author

Theoretical novel medical isotope production with deuterium-tritium fusion technology.

Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine·2025

Same author

Imaging PD-L1 in the brain-Journey from the lab to the clinic.

Neuro-oncology·2024

Same author

Immuno-PET Imaging of Tumour PD-L1 Expression in Glioblastoma.

Cancers·2023

Same author

Shortwave Infrared Imaging Enables High-Contrast Fluorescence-Guided Surgery in Neuroblastoma.

Cancer research·2023

Search research articles

Related Experiment Video

Updated: Jul 10, 2025

The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies

The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies

Published on: February 12, 2015

Radiolabeled Affibody Molecules for PET Imaging.

Chiara Da Pieve¹, Gabriela Kramer-Marek²

¹Preclinical Molecular Imaging, Division of Radiotherapy and Imaging, The Institute of Cancer Research, London, UK.

Methods in Molecular Biology (Clifton, N.J.)

|November 25, 2023

Summary

Radiolabeled affibody molecules are excellent molecular imaging probes due to their specific targeting. This study details methods for preparing affibody-chelator conjugates and radiolabeling them for preclinical and clinical applications.

Keywords:

18F-AlF 68Ga 89Zr Conjugation chelators

More Related Videos

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Published on: February 3, 2015

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

Related Experiment Videos

Last Updated: Jul 10, 2025

The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies

The Bioconjugation and Radiosynthesis of 89Zr-DFO-labeled Antibodies

Published on: February 12, 2015

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Harnessing the Bioorthogonal Inverse Electron Demand Diels-Alder Cycloaddition for Pretargeted PET Imaging

Published on: February 3, 2015

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Murine Lymphocyte Labeling by 64Cu-Antibody Receptor Targeting for In Vivo Cell Trafficking by PET/CT

Published on: April 29, 2017

Area of Science:

Bioconjugation Chemistry
Radiopharmaceutical Chemistry
Molecular Imaging

Background:

Affibody molecules offer advantages like ease of engineering, stability, and high specificity for molecular imaging.
Radiolabeled affibody probes are valuable tools in both preclinical research and clinical diagnostics.

Purpose of the Study:

To describe methods for preparing affibody-chelator conjugates.
To detail the subsequent radiolabeling of these conjugates using common radioisotopes.

Main Methods:

Preparation of affibody-chelator conjugates.
Radiolabeling using 18F-AlF, 68Ga, and 89Zr.

Main Results:

Successful preparation of affibody-chelator conjugates.
Efficient radiolabeling of conjugates with 18F-AlF, 68Ga, and 89Zr.

Conclusions:

The described methods facilitate the development of radiolabeled affibody probes.
These probes show promise for advanced molecular imaging applications.