The Basics of Visualizing, Analyzing, and Reporting Preclinical PET/CT Imaging Data

Area of Science:

• Preclinical Imaging
• Medical Physics
• Radiochemistry

Background:

• Positron Emission Tomography (PET) is a powerful, quantitative, and highly sensitive noninvasive imaging technique.
• PET is frequently combined with Computed Tomography (CT) or Magnetic Resonance Imaging (MRI) for anatomical context.
• Advancements in PET imaging have enhanced physiological and pathophysiological insights and therapeutic development.

Purpose of the Study:

• To provide a protocol for inexperienced users to perform preclinical PET/CT data analysis.
• To describe general principles and workflows for PET/CT image visualization and quantitative analysis.
• To offer recommendations for reporting preclinical PET/CT data.

Main Methods:

• The protocol details general principles and workflows for PET/CT data analysis.
• It covers visualization and quantitative analysis using popular software packages.
• Recommendations for reporting preclinical PET/CT data are presented, including examples.

Main Results:

• The protocol aims to guide users through complex PET/CT data analysis.
• It addresses potential pitfalls in PET image data analysis that can affect reporting and reproducibility.
• The study provides practical guidance for standardized reporting.

Conclusions:

• This protocol facilitates reproducible preclinical PET/CT data analysis for researchers.
• Adherence to recommended practices enhances the reliability of findings and reporting.
• Standardized analysis and reporting are crucial for advancing preclinical research.