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Host-pathogen interactions from a metabolic perspective: methods of investigation
Caroline Demangel1, Laura Surace2
1Institut Pasteur, Université Paris Cité, Inserm U1224, Immunobiology and Therapy Unit, Paris, France.
Microbes and Infection
|November 25, 2023
Summary
This review explores methods for studying how metabolism and immunity interact. Understanding this relationship is crucial for immune homeostasis in both health and disease, especially during infections.
Area of Science:
- Immunology
- Metabolic research
- Systems biology
Background:
- Immune homeostasis is critical for health and disease.
- Metabolism significantly influences immune cell function.
- The interplay between metabolism and immunity is complex and multifaceted.
Purpose of the Study:
- To review current methodologies for investigating the metabolism-immunity axis.
- To highlight techniques applicable across different biological scales (systemic, tissue, cellular).
- To focus on methods relevant to understanding immune responses during infection.
Main Methods:
- Systematic literature review of relevant research methodologies.
- Categorization of methods based on biological scale and application.
- Emphasis on techniques applicable to host-pathogen interactions.
Main Results:
- A comprehensive overview of diverse experimental approaches.
- Identification of key techniques for analyzing metabolic pathways in immune cells.
- Discussion of methods for assessing immune responses in the context of metabolic perturbations.
Conclusions:
- A robust toolkit exists to study metabolism-immunity interactions.
- Further methodological development is needed for a complete understanding.
- These methods are vital for advancing immunology and infectious disease research.
