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Updated: Jul 10, 2025

miRNA Expression Analyses in Prostate Cancer Clinical Tissues
11:29

miRNA Expression Analyses in Prostate Cancer Clinical Tissues

Published on: September 8, 2015

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CircTENM3 inhibites tumor progression via the miR-558/RUNX3 axis in prostate cancer

Lingxiang Lu1, Fei Wang2, Jianchun Chen1

  • 1Department of Urinary Surgery, Suzhou Ninth People's Hospital, Soochow University, Suzhou, Jiangsu, China.

Journal of Translational Medicine
|November 25, 2023
PubMed
Abstract

Insights

Circular RNA circTENM3 is downregulated in prostate cancer (PCa). Overexpressing circTENM3 inhibits PCa progression by sponging miR-558 to increase RUNX3 levels, offering a potential diagnostic and therapeutic target for PCa.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Prostate cancer (PCa) is a leading cancer in men globally.
  • Lack of effective diagnostic biomarkers and therapeutic targets hinders PCa treatment.
  • Circular RNAs show promise as biomarkers for various diseases, including cancer.

Purpose of the Study:

  • To identify a specific circular RNA (circRNA) as a diagnostic and therapeutic target for PCa.
  • To investigate the role and mechanism of circTENM3 in prostate cancer.

Main Methods:

  • Quantitative PCR (qPCR) to assess circTENM3 expression in PCa.
  • In vitro assays (CCK8, EdU, Scratch, Transwell) to evaluate circTENM3's function in PCa cells.
  • Mechanism exploration using RIP, RNA-pull down, and luciferase reporter assays.
  • In vivo gain-of-function analysis to confirm circTENM3's role in PCa progression.

Main Results:

  • circTENM3 expression was significantly downregulated in PCa tissues.
  • Overexpression of circTENM3 inhibited PCa cell proliferation, migration, and invasion in vitro.
  • circTENM3 acts as a molecular sponge for miR-558, leading to increased RUNX3 expression.
  • Gain-of-function studies confirmed circTENM3's inhibitory effect on PCa progression in vivo.

Conclusions:

  • circTENM3 plays a protective role in prostate cancer.
  • The circTENM3/miR-558/RUNX3 axis is a key pathway in PCa pathogenesis.
  • circTENM3 represents a potential diagnostic biomarker and therapeutic target for PCa.
Keywords:
PCaRUNX3circTENM3miR-558

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