Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.3K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.3K
Immunogold Electron Microscopy01:20

Immunogold Electron Microscopy

4.0K
Immunoelectron microscopy utilizes immunogold labeling of endogenous proteins with specific antibodies to detect and localize these proteins in cells and tissues. The procedure provides insights into the distribution and quantification of protein under different stimulation conditions offering clues about their functions. Conjugating highly electron-dense gold particles with primary or secondary antibodies allow antigen detection on and within cells, with high resolution and specificity.
4.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prediction Model for Children With Anaphylaxis Who May Not Require Emergency Department Care: A Multicenter Retrospective Cohort Study.

The journal of allergy and clinical immunology. In practice·2026
Same author

Measurement of atomic scattering factors by cryoelectron microscopy.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Correction: A feature-based qualitative assessment of smoking cessation mobile applications.

PLOS digital health·2026
Same author

A tale of two apps: preliminary usability testing of smoking cessation mobile applications among individuals with low socioeconomic status who smoke cigarettes.

BMC medical informatics and decision making·2026
Same author

Perceptions of User-Generated Content as a Source of Health Messages in Smoking Cessation Mobile Interventions: Focus Group Study.

JMIR human factors·2025
Same author

Acceptability of a carbon monoxide monitoring feature of a smoking cessation mobile application: a virtual focus group study.

BMJ public health·2025

Related Experiment Video

Updated: Jul 10, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.2K

Accurate magnification determination for cryoEM using gold.

Joshua L Dickerson1, Erin Leahy1, Mathew J Peet1

  • 1MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.

Ultramicroscopy
|November 26, 2023
PubMed
Summary

Accurately determining magnified pixel size in cryo-electron microscopy (cryoEM) is crucial for high-resolution imaging. A new, simple method using gold crystal lattice spacing offers precise, rapid magnification calibration for improved data analysis and model building.

Keywords:
CryoEMElectron cryomicroscopyGoldMagnification calibrationPixel sizeProtein structureSingle-particle reconstruction

More Related Videos

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

13.3K
Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

4.5K

Related Experiment Videos

Last Updated: Jul 10, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
09:49

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

5.2K
A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion
13:43

A Robust Single-Particle Cryo-Electron Microscopy cryo-EM Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

13.3K
Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition
08:16

Strategies for Optimization of Cryogenic Electron Tomography Data Acquisition

Published on: March 19, 2021

4.5K

Area of Science:

  • Cryo-electron microscopy (cryoEM)
  • Structural biology
  • Materials science

Background:

  • Accurate magnification calibration is essential for high-resolution single-particle cryoEM.
  • Precise pixel size determination directly impacts resolution and model building accuracy.

Purpose of the Study:

  • To develop a simple, rapid, and accurate method for determining absolute magnification in electron cryomicroscopes.
  • To establish a reliable calibration standard for cryoEM data acquisition and processing.

Main Methods:

  • Utilizing the known atomic lattice spacings of gold crystals as an absolute reference.
  • Applying the method for both room temperature and cryogenic imaging conditions.
  • Comparing the new method with existing magnification calibration techniques.

Main Results:

  • Achieved magnification determination with a precision of less than 0.5%.
  • Demonstrated comparable accuracy to other methods despite the procedure's simplicity.
  • Validated the method using readily available gold test specimens.

Conclusions:

  • The gold lattice spacing method provides a definitive reference for cryoEM magnification calibration.
  • Simplifies combining datasets from different microscopes and detectors.
  • Enhances the accuracy of contrast transfer function determination and ex post facto pixel size estimation using the open-source magCalEM program.