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RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...

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Updated: Jul 10, 2025

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

Single-cell RNA sequencing to understand host-virus interactions.

Jia-Tong Chang¹, Li-Bo Liu¹, Pei-Gang Wang¹

¹Department of Microbiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China.

Virologica Sinica

|November 26, 2023

Summary

Single-cell RNA sequencing (scRNA-seq) offers a powerful way to study viruses and the immune system at the cellular level. This technology enhances our understanding of host-virus interactions and aids in developing new therapies.

Keywords:

COVID-19 Flavivirus Host-virus interaction Single-cell RNA sequencing (scRNA-seq)

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Last Updated: Jul 10, 2025

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

High-Throughput Transcriptome Analysis for Investigating Host-Pathogen Interactions

Published on: March 5, 2022

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors

Published on: September 13, 2018

Area of Science:

Virology
Immunology
Genomics
Bioinformatics

Background:

Single-cell RNA sequencing (scRNA-seq) provides unprecedented resolution for analyzing cellular gene expression.
Understanding host-virus interactions is crucial for combating infectious diseases.

Purpose of the Study:

To review current scRNA-seq technologies and their applications in virology.
To highlight the potential of scRNA-seq in advancing our knowledge of immune responses to viral infections.
To explore how scRNA-seq can inform the development of novel therapeutic strategies.

Main Methods:

Comprehensive literature review of scRNA-seq methodologies.
Analysis of published studies utilizing scRNA-seq in virological research.
Synthesis of findings related to immune atlas, host-virus interactions, and immune repertoire.

Main Results:

scRNA-seq enables detailed profiling of host and viral transcripts.
It offers insights into cellular-level immune responses and viral dynamics.
The technology reveals complex host-virus interactions and immune repertoire characteristics.

Conclusions:

scRNA-seq is a transformative tool for virological research.
It significantly enhances understanding of immune responses and viral pathogenesis.
Future applications promise accelerated discovery of effective antiviral therapies.