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Parameter evaluation for developing phosphate-solubilizing Bacillus inoculants.

Daniel Bini1, Bianca Braz Mattos2, José Edson Fontes Figueiredo3

  • 1Embrapa Milho E Sorgo, Sete Lagoas, MG, 35701-970, Brazil.

Brazilian Journal of Microbiology : [Publication of the Brazilian Society for Microbiology]
|November 26, 2023
PubMed
Summary
This summary is machine-generated.

Bacillus subtilis (B70) and Bacillus pumilus (B32) were evaluated as bacterial inoculants for maize. Activated charcoal effectively preserved bacterial viability, with B70 showing superior phosphorus solubilization and yield enhancement in field trials.

Keywords:
Zea mays LMaize yieldPhosphate fertilizersPhosphate-solubilizing bacteriaShelf life

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Area of Science:

  • Agricultural Microbiology
  • Plant Nutrition
  • Soil Science

Background:

  • Bacterial inoculants enhance agricultural productivity but require rigorous evaluation.
  • Optimizing inoculant formulation is crucial for field efficacy.
  • Phosphorus availability is a key limiting factor in maize production.

Purpose of the Study:

  • To assess Bacillus subtilis (B70) and Bacillus pumilus (B32) for maize phosphorus (P) enhancement.
  • To evaluate carrier materials and storage conditions for inoculant stability.
  • To determine the field performance of selected inoculants with rock phosphate.

Main Methods:

  • In vitro screening for phosphate solubilization, mineralization, root adherence, and shelf-life in various carriers (CS, CMC, peat, AC).
  • Field trials with maize (Zea mays L.) using rock phosphate (RP) and RP/triple superphosphate (TS) mixtures.
  • Assessment of bacterial survival, P availability, maize yield, and grain P accumulation.

Main Results:

  • Bacillus subtilis (B70) excelled in P solubilization and mineralization, showing better stability in carriers than B32.
  • Activated charcoal (AC) proved the most effective carrier for maintaining bacterial viability.
  • The B70 inoculant with rock phosphate significantly improved maize yield (6532 kg ha⁻¹) and grain P accumulation (15.95 kg ha⁻¹).

Conclusions:

  • Inoculant formulation with activated charcoal and Bacillus subtilis (B70) is a viable strategy.
  • This approach enhances soil phosphorus mobilization and maize productivity.
  • Optimized bacterial inoculants offer a sustainable solution for improving crop nutrition.