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Determining protein-drug binding can be achieved through indirect and direct methods, each providing valuable insights into the interaction between proteins and drugs.
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Related Experiment Video

Updated: Jul 10, 2025

Determination of High-affinity Antibody-antigen Binding Kinetics Using Four Biosensor Platforms
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TitrationAnalysis: a tool for high throughput binding kinetics data analysis for multiple label-free platforms.

Kan Li1,2, Richard H C Huntwork1,2, Gillian Q Horn1,2

  • 1Center for Human Systems Immunology, Duke University, Durham, North Carolina, 27701, USA.

Gates Open Research
|November 27, 2023
PubMed
Summary
This summary is machine-generated.

A new Mathematica package, TitrationAnalysis, efficiently analyzes label-free binding kinetics data from SPR and BLI platforms. This tool accurately determines association and dissociation rate constants for biomolecular interactions.

Keywords:
Biolayer InterferometrySurface Plasmon Resonanceantibody bindinghigh-throughput kinetics analysisnon-linear curve fitting

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Area of Science:

  • Biophysics
  • Biochemistry
  • Computational Biology

Background:

  • Label-free techniques like Surface Plasmon Resonance (SPR) and Biolayer Interferometry (BLI) are crucial for studying bimolecular interactions.
  • Analyzing binding kinetics data from these platforms can be complex and time-consuming.

Purpose of the Study:

  • To develop a high-throughput analysis tool, TitrationAnalysis, for efficient and accurate analysis of SPR and BLI binding kinetics data.
  • To provide a user-friendly solution requiring minimal scripting knowledge.

Main Methods:

  • Development of the TitrationAnalysis package for Mathematica.
  • Utilizing Mathematica's non-linear curve-fitting module for data analysis.
  • Application to antibody-antigen binding data from Biacore T200, Carterra LSA, and ForteBio Octet Red384 platforms.

Main Results:

  • TitrationAnalysis accurately estimates association (k_a) and dissociation (k_d) rate constants, and apparent dissociation constants (K_d).
  • Results closely matched those obtained from commercial analysis software specific to the instruments.
  • The tool demonstrated high throughput and automatic fitting capabilities.

Conclusions:

  • TitrationAnalysis offers a powerful, versatile, and accurate alternative for biomolecular interaction characterization.
  • The customizable output facilitates downstream data quality control, including Good Clinical Laboratory Practice operations.
  • This tool enhances the efficiency of analyzing label-free binding kinetics data across multiple platforms.