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Simplified Intrafemoral Injections Using Live Mice Allow for Continuous Bone Marrow Analysis
Published on: November 10, 2023
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Simplified Intrafemoral Injections Using Live Mice Allow for Continuous Bone Marrow Analysis.
Yusuke Nakauchi1, Asiri Ediriwickrema1, Daniel Martinez-Krams1
1Department of Medicine, Division of Hematology, Cancer Institute, and Institute for Stem Cell Biology and Regenerative Medicine, Stanford University School of Medicine.
Journal of Visualized Experiments : Jove
|November 27, 2023
Summary
Intrafemoral injection in mice improves hematopoietic stem and progenitor cell (HSPC) engraftment. This method allows serial bone marrow analysis and xenograft creation without sacrificing the animals.
Area of Science:
- Hematology
- Transplantation Biology
- Animal Models
Background:
- Hematopoietic cell transplantation is complex in humans.
- Intravenous or intrafemoral (IF) injections are common in murine models.
- IF injection enhances hematopoietic stem and progenitor cell (HSPC) seeding efficiency.
Purpose of the Study:
- To describe a detailed step-by-step technical procedure for IF injection and bone marrow (BM) aspiration in mice.
- To enable serial characterization of cells within the BM.
- To facilitate transplantation of low cell numbers and creation of xenografts.
Main Methods:
- Detailed protocol for intrafemoral (IF) injection in mice.
- Serial bone marrow (BM) aspiration technique.
- Application in hematopoietic stem and progenitor cell (HSPC) transplantation and xenograft generation.
Main Results:
- Successful transplantation of valuable samples with low cell numbers.
- Enhanced engraftment compared to intravenous injection.
- Facilitation of xenograft creation for pathological analysis.
Conclusions:
- IF injection and serial BM aspiration provide essential access to the BM compartment for studying hematopoiesis.
- This method allows prospective retrieval and characterization of BM-enriched cells, including HSPCs, without animal sacrifice.
- Crucial for research involving low cell numbers and xenograft models.
