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Analyzing the Effects of Stromal Cells on the Recruitment of Leukocytes from Flow
Published on: January 7, 2015
Tumor necrosis factor-induced alterations in circulating leukocyte populations
Biochemical and Biophysical Research Communications
|December 15, 1986
Summary
Intravenous administration of tumor necrosis factor (TNF) in mice caused significant lymphopenia and neutrophilia. These immune cell changes peaked at 4 hours post-injection, demonstrating TNF's potent effects on blood constituents.
Area of Science:
- Immunology
- Hematology
Background:
- Tumor necrosis factor (TNF) is a cytokine with diverse biological functions.
- Understanding TNF's impact on immune cells is crucial for therapeutic applications.
Purpose of the Study:
- To investigate the effects of intravenous human recombinant TNF on immune cell populations in CBA/J mice.
Main Methods:
- Intravenous administration of human recombinant TNF to CBA/J mice.
- Monitoring of peripheral blood immune cell populations at various time points post-injection.
- Implementation of extensive controls to exclude LPS contamination.
Main Results:
- Significant lymphopenia and neutrophilia observed, peaking at 4 hours post-injection.
- Both relative and absolute lymphopenia and neutrophilia were recorded.
- Neutrophilia involved both immature and mature neutrophils.
- Concomitant lymphopenia and neutrophilia resulted in no change in total white blood cell count.
Conclusions:
- Intravenous administration of human recombinant TNF profoundly impacts peripheral blood immune cell populations.
- The study highlights the potent hematological effects of TNF.
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