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Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

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Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
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Electron Microscope Tomography and Single-particle Reconstruction01:07

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Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
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Related Experiment Video

Updated: Jul 9, 2025

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope
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Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

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Structure determination by cryoEM at 100 keV.

Greg McMullan1, Katerina Naydenova1, Daniel Mihaylov1

  • 1Medical Research Council (MRC) Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.

Proceedings of the National Academy of Sciences of the United States of America
|November 27, 2023
PubMed
Summary
This summary is machine-generated.

A new, affordable electron cryomicroscopy instrument enables rapid, high-resolution imaging of biological molecules. This breakthrough in structural biology makes complex molecular structure determination accessible and fast for a wide range of research problems.

Keywords:
100 keVTEMcryoEMelectron microscopystructure determination

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Area of Science:

  • Structural Biology
  • Biophysics
  • Biochemistry

Background:

  • Electron cryomicroscopy (CryoEM) is a powerful technique for determining biological molecule structures.
  • Current CryoEM methods face limitations in accessibility, specimen preparation, and cost, hindering widespread adoption.

Purpose of the Study:

  • To develop and validate a novel, purpose-built electron cryomicroscopy instrument.
  • To significantly reduce the cost and complexity of biological structure determination.

Main Methods:

  • A new 100 keV electron microscope incorporating advanced electron optics, detection, and processing.
  • Demonstration of atomic resolution imaging on gold test specimens.
  • Structure determination of eleven diverse biological specimens (140 kDa to 2 MDa) using reduced data.

Main Results:

  • The instrument achieves theoretical performance limits, enabling atomic resolution imaging.
  • Biological molecular structure determination is achieved within hours.
  • Successful determination of structures for eleven specimens with significantly less data than typically required.

Conclusions:

  • The developed CryoEM instrument makes high-resolution structure determination fast, simple, and cost-effective.
  • This technology has the potential to expand structural biology to previously intractable problems.
  • Enables high-efficiency, on-the-spot imaging for broader accessibility in structural biology research.