Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.2K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.2K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.6K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.6K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

743
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
743
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.2K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autoantigen mRNA-LNP Vaccination Drives Therapeutic Efficacy in Preclinical Models for Autoimmunity.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

ScopeViewer: a browser-based solution for visualizing large biological images.

GigaScience·2026
Same author

Predictive Biomarkers for Immune Checkpoint Inhibitor Efficacy: Challenges, Innovations, and a Pathway to Precision Medicine in the Era of Cancer Immunotherapy.

Clinical chemistry·2026
Same author

Oligonucleotide genetics for <i>Pseudomonas aeruginosa</i> enables high throughput hypomorph screening.

bioRxiv : the preprint server for biology·2026
Same author

Reciproc and XP-endo Shaper Outperform WaveOne Gold in Apical Debris Removal: A Micro-CT Study in 3D-Printed Molars.

Current medical science·2026
Same author

Host-Induced Gene Silencing of <i>SmDSR32</i> Enhances Wheat Defense Against <i>Sitobion miscanthi</i>.

Current issues in molecular biology·2026

Related Experiment Video

Updated: Jul 9, 2025

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
06:47

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Published on: July 30, 2015

10.8K

OVOL2 sustains postnatal thymic epithelial cell identity.

Xue Zhong1, Nagesh Peddada1, Jianhui Wang1

  • 1Center for the Genetics of Host Defense, University of Texas Southwestern Medical Center, Dallas, TX, 75390-8505, USA.

Nature Communications
|November 27, 2023
PubMed
Summary

Ovol2 protein is crucial for maintaining thymic epithelial cell (TEC) identity and function postnatally. Its deficiency leads to T cell lymphopenia by disrupting epigenetic regulation and promoting a mesenchymal state in TECs.

More Related Videos

Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids
03:31

Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids

Published on: October 4, 2024

1.6K
Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
09:41

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms

Published on: July 26, 2024

777

Related Experiment Videos

Last Updated: Jul 9, 2025

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression
06:47

3D Organotypic Co-culture Model Supporting Medullary Thymic Epithelial Cell Proliferation, Differentiation and Promiscuous Gene Expression

Published on: July 30, 2015

10.8K
Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids
03:31

Author Spotlight: Advancing Thymic Epithelial Cells and T-Cell Research with Human Thymic Organoids

Published on: October 4, 2024

1.6K
Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms
09:41

Preparation of Single-Cell Suspension of Mouse Thymic Epithelial Cells and Staining of Intracellular Molecules for Flow Cytometric AnalysisMechanisms

Published on: July 26, 2024

777

Area of Science:

  • Immunology
  • Developmental Biology
  • Epigenetics

Background:

  • Distinct molecular pathways regulate embryonic versus postnatal thymic epithelial cell (TEC) development.
  • Understanding postnatal TEC maintenance is key to addressing thymic involution.

Purpose of the Study:

  • To identify the molecular mechanisms maintaining postnatal TEC numbers and function.
  • To elucidate the role of Ovol2 in TEC identity and epigenetic regulation.

Main Methods:

  • Utilized a missense Ovol2 allele (C120Y) in mice, with ubiquitous or TEC-specific expression.
  • Analyzed T cell development, TEC populations (medullary and cortical), and epigenetic modifications.
  • Investigated the interaction of Ovol2 with the BRAF-HDAC complex, including RCOR1-LSD1.

Main Results:

  • Ovol2 deficiency in mice caused lymphopenia, characterized by loss of medullary TECs and dysfunction of cortical TECs.
  • OVOL2-deficient TECs exhibited a switch from epithelial to mesenchymal identity.
  • Ovol2 was shown to inhibit the BRAF-HDAC complex, disrupting RCOR1-LSD1 interaction and preventing LSD1-mediated H3K4me2 demethylation, thus maintaining chromatin accessibility for epithelial gene expression.

Conclusions:

  • Ovol2 is essential for enforcing TEC identity by controlling the epigenetic landscape postnatally.
  • This Ovol2-dependent mechanism offers insights into thymic involution and T cell development.