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Published on: August 19, 2016
Distribution characteristics of circulating B cell subpopulations in patients with chronic kidney disease
Xuya Chen1, Haoyang Guo1, Danxia Jin1
1Clinical Laboratory, Affiliated Dongyang Hospital of Wenzhou Medical University, 60 West Wuning Road, Dongyang, 322100, Zhejiang, China.
Insights
Chronic kidney disease (CKD) alters circulating B cell subpopulations, with advanced stages showing decreased transitional and CD5+ B cells. Haemodialysis effects on these immune cells in end-stage renal disease require further investigation.
Area of Science:
- Immunology
- Nephrology
- Cell Biology
Background:
- Chronic kidney disease (CKD) is associated with immune dysregulation.
- Understanding B cell dynamics in CKD is crucial for managing immune homeostasis.
- The impact of renal function decline on B cell subpopulations remains incompletely understood.
Purpose of the Study:
- To compare B cell subpopulations across different CKD stages.
- To investigate the effects of haemodialysis (HD) on B cell profiles in end-stage renal disease (ESRD).
- To evaluate the relationship between renal function and immune balance in CKD patients.
Main Methods:
- Flow cytometry analysis of circulating B cell subpopulations.
- Inclusion of 197 CKD patients (stages I-V, including HD patients) and 77 healthy controls.
- Comparison of B cell distribution between CKD stages, healthy controls, and pre/post-HD.
Main Results:
- CKD stage V patients exhibited decreased transitional B cells and CD5+ B cells, alongside increased double-negative (DN) B cells compared to controls.
- Advanced CKD stages showed a significant reduction in the absolute counts of various B cell subpopulations.
- B cell subpopulation distribution was significantly altered with CKD progression; HD effects on DN and CD5+ B cells were inconsistent.
Conclusions:
- Circulating B cell subpopulations are significantly altered in CKD and associated with disease progression.
- Immune homeostasis is disrupted in CKD, with specific changes in B cell subsets.
- Further research into HD's impact on B cell profiles is warranted for clinical implications.
Abstract:
This study compared the levels of circulating B cell subpopulations in patients with different stages of chronic kidney disease (CKD), investigated the effects of haemodialysis (HD) on the B cell-related immune spectrum in patients with end-stage renal disease, and evaluated the link between renal function and immune homeostasis. Overall, 197 patients with CKD (158 non-dialysis patients with CKD stages I-V and 39 end-stage patients undergoing maintenance HD) and 77 healthy controls were included. Compared to healthy controls, patients with CKD stages I-II showed no significant differences except for the proportion of transitional B cells; patients with CKD stage V showed a significant decrease in the proportions of transitional B cells and CD5+ B cells and a significant increase in double-negative (DN) B cells. Compared with early-stage patients with CKD, the absolute count of various B cell subpopulations in advanced-stage patients with CKD showed a significant decrease. The distribution of circulating B cell subpopulations in patients with CKD was significantly altered and was associated with CKD progression. Furthermore, the proportion of DN B cells and CD5+ B cells was inconsistent pre- and post-HD. This in-depth study of the immune status of patients with CKD may have important clinical value.
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