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Related Experiment Video

Updated: Jul 9, 2025

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Direct Imaging of Laser-driven Ultrafast Molecular Rotation

Published on: February 4, 2017

Optical constraints on two-photon voltage imaging.

F Phil Brooks¹, Hunter C Davis¹, J David Wong-Campos¹

¹Department of Chemistry and Chemical Biology, Harvard University.

Biorxiv : the Preprint Server for Biology

|November 28, 2023

Summary

This summary is machine-generated.

Two-photon (2P) voltage imaging requires significantly more power and can image fewer cells than one-photon (1P) imaging. Achieving high-SNR 2P imaging of many neurons at depth necessitates improved genetically encoded voltage indicators (GEVIs) or novel imaging techniques.

Keywords:

Shot noise Two-photon Voltage imaging

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Area of Science:

Neuroscience
Optical Imaging
Biophysics

Background:

Genetically encoded voltage indicators (GEVIs) are crucial for in vivo neural circuit studies.
The comparative performance of one-photon (1P) and two-photon (2P) voltage imaging requires detailed characterization.

Conclusions:

Existing 2P GEVIs face a critical trade-off between shot noise and photodamage due to stringent photon requirements.
Imaging hundreds of neurons with high SNR at depths >300 μm necessitates substantial GEVI improvements or new imaging modalities.