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Updated: Jul 9, 2025
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Deep-Tissue Three-Photon Fluorescence Microscopy in Intact Mouse and Zebrafish Brain
Published on: January 13, 2022
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A Large Field-of-view, Single-cell-resolution Two- and Three-Photon Microscope for Deep Imaging
Aaron T Mok1,2, Tianyu Wang1, Shitong Zhao1
1School of Applied Engineering Physics, Cornell University, NY, USA.
Biorxiv : the Preprint Server for Biology
|November 28, 2023
Summary
Researchers developed new three-photon microscopy techniques to overcome field-of-view limitations for deep brain imaging. This allows unprecedented large-scale, high-speed neuronal activity mapping in scattering tissues.
Area of Science:
- Neuroscience
- Biomedical Engineering
- Optical Imaging
Background:
- In vivo imaging of neural networks is crucial for understanding brain function.
- Multiphoton microscopy advances speed, field of view, and depth but faces limitations in scattering tissues.
- Field of view in deep-tissue imaging decreases exponentially with depth due to thermal damage concerns.
Conclusions:
- The developed innovations overcome limitations in deep-tissue multiphoton imaging.
- These techniques facilitate large-field-of-view, system-level neural circuit research.
- The methods are adaptable to existing multiphoton microscopes.
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