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A Method for Analyzing AFM Force Mapping Data Obtained from Soft Tissue Cryosections
Cydney A Wong1, Nina Sara Fraticelli Guzmán2, A Thomas Read1
1Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology & Emory University, Atlanta, GA, USA.
Biorxiv : the Preprint Server for Biology
|November 28, 2023
Summary
This study introduces a new Atomic Force Microscopy (AFM) method for analyzing soft tissue mechanics using cryosections. This robust pipeline enhances the reliability of mechanical property measurements in heterogeneous biological samples.
Area of Science:
- Biophysics
- Materials Science
- Ophthalmology
Background:
- Atomic Force Microscopy (AFM) is crucial for evaluating biological sample mechanics.
- Interpreting AFM data from heterogeneous whole tissues presents significant challenges.
- Robust methods are needed to accurately assess soft tissue mechanical properties.
Conclusions:
- The proposed AFM methodology provides a reliable approach for characterizing soft tissue mechanics.
- This pipeline can be widely adopted for analyzing cryosectioned soft tissues.
- The findings contribute to a better understanding of tissue biomechanics.
