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Related Concept Videos

Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

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Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
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Phosphorylation01:02

Phosphorylation

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The addition or removal of phosphate groups from proteins is the most common chemical modification that regulates cellular processes. These modifications can affect the structure, activity, stability, and localization of proteins within cells as well as their interactions with other proteins.
During phosphorylation, protein kinases transfer the terminal phosphate group of ATP to specific amino acid side chains of substrate proteins. Serine, threonine, and tyrosine are the most commonly...
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Proteomics01:33

Proteomics

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A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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Related Experiment Video

Updated: Jul 9, 2025

Oligopeptide Competition Assay for Phosphorylation Site Determination
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Oligopeptide Competition Assay for Phosphorylation Site Determination

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Proteome-wide base editor screens to assess phosphorylation site functionality in high-throughput.

Patrick H Kennedy, Amin Alborzian Deh Sheikh, Matthew Balakar

    Biorxiv : the Preprint Server for Biology
    |November 28, 2023
    PubMed
    Summary

    Researchers developed a new method to map signaling pathways by linking post-translational modifications (PTMs) to gene expression. This approach reveals how thousands of PTMs fine-tune cellular functions and transcriptional responses.

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    Area of Science:

    • Cellular signaling
    • Molecular biology
    • Genetics

    Background:

    • Cellular signaling pathways are complex, involving thousands of post-translational modifications (PTMs) that regulate gene expression.
    • Current technologies are limited in their ability to link PTMs to genetic circuits at scale.
    • Understanding PTMs is crucial for deciphering cellular functions.

    Conclusions:

    • PTM-centric base editor screening is a powerful platform for dissecting PTM function in signaling pathways.
    • This technology enables large-scale analysis of PTMs' role in cellular regulation.
    • The findings provide new insights into the intricate control of gene expression by PTMs.