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Updated: Jul 9, 2025
09:16
Oligopeptide Competition Assay for Phosphorylation Site Determination
Published on: May 18, 2017
8.5K
Proteome-wide base editor screens to assess phosphorylation site functionality in high-throughput.
Biorxiv : the Preprint Server for Biology
|November 28, 2023
Summary
Researchers developed a new method to map signaling pathways by linking post-translational modifications (PTMs) to gene expression. This approach reveals how thousands of PTMs fine-tune cellular functions and transcriptional responses.
Area of Science:
- Cellular signaling
- Molecular biology
- Genetics
Background:
- Cellular signaling pathways are complex, involving thousands of post-translational modifications (PTMs) that regulate gene expression.
- Current technologies are limited in their ability to link PTMs to genetic circuits at scale.
- Understanding PTMs is crucial for deciphering cellular functions.
Conclusions:
- PTM-centric base editor screening is a powerful platform for dissecting PTM function in signaling pathways.
- This technology enables large-scale analysis of PTMs' role in cellular regulation.
- The findings provide new insights into the intricate control of gene expression by PTMs.
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