Rapid fingerprinting of bacterial species using nanocavities created on screen-printed electrodes modified by...

Rapid and precise identification of infectious microorganisms is important across a range of applications where microbial contamination can cause serious issues ranging from microbial resistance to corrosion. In this paper a screen-printed, polymeric β-cyclodextrin (β-CD) modified electrode, affording nanocavities for inclusion of the analytes, is shown as a disposable sensor capable of identifying bacteria by their metabolites. Three bacterial species were tested: two from the Pseudomonas genus, Pseudomonas fluorescens (P. fluorescens) and Pseudomonas aeruginosa (P. aeruginosa), and Serratia marcescens (S. marcescens), a member of the family, Enterobacteriaceae. On biofilm formation each species gave distinct, reproducible, redox fingerprints with a detection limit of 4 × 10^-8 M. Square wave adsorptive stripping voltammetry (SWAdSV) was used for detection. Scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques were used to characterize the morphology and electrical conductivity of the modified electrode. In comparison to the bare screen-printed electrode, the modified electrode showed a considerably higher performance and offered an excellent sensitivity along with a relatively fast analysis time.