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Harnessing multiplex crRNA enables an amplification-free/CRISPR-Cas12a-based diagnostic methodology for Nosema

Huarui Zhang1,2, Huijuan Zhao1,2, Lu Cao1,2

  • 1State Key Laboratory of Resource Insects, Southwest University , Chongqing, China.

Microbiology Spectrum
|November 28, 2023
PubMed
Abstract

Keywords:
CRISPR/Cas12aNosema bombycisamplification-free DNA diagnosticmultiplex crRNApathogen detection

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CRISPR and crRNAs02:53

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...