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Updated: Aug 12, 2026
Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
Identification and nucleotide sequencing of a novel partitivirus derived from Rhizoctonia solani AG-4 HG III isolate
Rui Shi1, Bengzha Dong1, Qiaodi Wang1
1College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, Yunnan, China.
Abstract:
Rhizoctonia solani is a widely disseminated phytopathogen that is found in the soil and is capable of harming many important species of crops. Here, analysis of the R. solani AG-4 HG III strain A14 led to the identification of a novel mycovirus assigned the tentative name "Rhizoctonia solani partitivirus A14" (RsPV-A14), which was subjected to sequencing and associated analyses. This approach revealed that RsPV-A14 harbored two dsRNA segments, 2022 bp (dsRNA1) and 1905 bp (dsRNA2) in length. dsRNA1 was found to contain a single open reading frame (ORF1) that codes for a 622-amino-acid protein with conserved RNA-dependent RNA polymerase (RdRp) motifs, and dsRNA2 was found to contain an ORF (ORF2) that is predicted to code for a 558-amino-acid capsid protein (CP). BLASTp analysis using the putative RdRp of RsPV-A14 showed sequence similarity to partitiviruses, including Rosellinia necatrix partitivirus 7 (50.53% identity), an unclassified partitivirus. Phylogenetic analysis based on RdRp protein sequences suggested that RsPV-A14 is a novel member of the family Partitiviridae.
Insights
A novel mycovirus, Rhizoctonia solani partitivirus A14 (RsPV-A14), was identified in the phytopathogen Rhizoctonia solani. This discovery expands our understanding of fungal viruses and their genetic diversity.
Area of Science:
- Mycology
- Virology
- Plant Pathology
Background:
- Rhizoctonia solani is a significant soil-borne phytopathogen affecting numerous crops.
- Understanding fungal viruses is crucial for managing plant diseases.
Purpose of the Study:
- To identify and characterize novel mycoviruses associated with Rhizoctonia solani.
- To investigate the genetic makeup and phylogenetic placement of the newly discovered virus.
Main Methods:
- Isolation and sequencing of double-stranded RNA (dsRNA) segments from Rhizoctonia solani AG-4 HG III strain A14.
- Bioinformatic analysis, including open reading frame (ORF) prediction and BLASTp searches.
- Phylogenetic analysis based on conserved RNA-dependent RNA polymerase (RdRp) protein sequences.
Main Results:
- Identification of a novel mycovirus, tentatively named Rhizoctonia solani partitivirus A14 (RsPV-A14).
- RsPV-A14 possesses two dsRNA segments (dsRNA1: 2022 bp, dsRNA2: 1905 bp).
- Sequence analysis revealed ORFs encoding a putative RNA-dependent RNA polymerase (RdRp) and a capsid protein (CP), with RdRp showing similarity to known partitiviruses.
Conclusions:
- RsPV-A14 represents a novel species within the family Partitiviridae.
- The characterization of RsPV-A14 contributes to the knowledge of fungal virus diversity and evolution.
- This finding may have implications for understanding the biology and pathogenicity of Rhizoctonia solani.
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