Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
Microbial Growth Measurement: Indirect Methods01:27

Microbial Growth Measurement: Indirect Methods

Estimating microbial growth is essential for understanding population dynamics and environmental adaptations. Indirect methods provide valuable insights by measuring parameters such as turbidity, metabolic activity, and biomass, enabling efficient and reproducible assessments.During exponential growth, microbial cells scatter light proportionally to their biomass, a principle used in turbidity measurements. About one million cells per milliliter produce detectable scattering, which a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multi-omic analysis reveals Wharton's jelly mesenchymal stem cells shift the pro-inflammatory phenotype of fibroblast subpopulation to induce skin rejuvenation.

Cell & bioscience·2026
Same author

IGFBP5 Restores Endometrial Receptivity and Rescues Implantation Failure in Polycystic Ovary Syndrome.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Molecular Mechanisms of Liraglutide Aggregation Induced by Dual Air-Water and Silicone-Oil-Water Interfacial Stress.

Molecular pharmaceutics·2026
Same author

4Pi stimulated Raman scattering for label-free super-resolution chemical imaging.

Science advances·2026
Same author

Label-Free Longitudinal Imaging of Single Cell Drug Response with a 3D-Printed Cell Culture Platform.

Analytical chemistry·2025
Same author

4-Pi Stimulated Raman Scattering for Label-free Super-resolution Chemical Imaging.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Jul 2, 2026

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
11:19

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

Published on: July 3, 2017

8.2K

Measuring Drug Response with Single-Cell Growth Rate Quantification.

Fiona Xi Xu1, Ruibing Wu1, Kailun Hu1

  • 1Department of Chemistry, University of Washington, Seattle, Washington 98195, United States.

Analytical Chemistry
|November 28, 2023
PubMed
Summary

This study introduces a novel label-free microscopy technique to measure single-cell growth rates in 2D and 3D. This method enables rapid, single-cell drug sensitivity testing, crucial for understanding cancer drug resistance.

More Related Videos

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression
12:52

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

Published on: April 18, 2021

4.8K
Comprehensive Analysis of Drug Response using the FLICK Assay
09:42

Comprehensive Analysis of Drug Response using the FLICK Assay

Published on: June 6, 2025

90

Related Experiment Videos

Last Updated: Jul 2, 2026

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth
11:19

ODELAY: A Large-scale Method for Multi-parameter Quantification of Yeast Growth

Published on: July 3, 2017

8.2K
High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression
12:52

High-Throughput Live Imaging of Microcolonies to Measure Heterogeneity in Growth and Gene Expression

Published on: April 18, 2021

4.8K
Comprehensive Analysis of Drug Response using the FLICK Assay
09:42

Comprehensive Analysis of Drug Response using the FLICK Assay

Published on: June 6, 2025

90

Area of Science:

  • Biophysics
  • Cell Biology
  • Cancer Research

Background:

  • Intratumoral heterogeneity drives cancer drug resistance.
  • Single-cell drug response monitoring is essential for effective cancer treatment.
  • Current methods for quantifying single-cell growth are limited to 2D cultures.

Purpose of the Study:

  • To develop a label-free technique for measuring 2D and 3D single-cell growth rates.
  • To enable rapid, single-cell drug sensitivity assays.
  • To investigate the impact of various chemotherapeutic drugs on cell growth and morphology.

Main Methods:

  • Utilized ratiometric stimulated Raman scattering (SRS) microscopy.
  • Employed deuterated amino acids (leucine, isoleucine, valine) as tracers.
  • Measured C-D and C-H SRS signals to determine single-cell growth rates in 2D and 3D cultures.

Main Results:

  • Accurately quantified 2D and 3D single-cell growth rates using label-free SRS microscopy.
  • Achieved single-cell drug sensitivity measurements within 12 hours.
  • Examined the effects of kinase inhibitors, mitotic inhibitors, and topoisomerase II inhibitors on cell growth and morphology.

Conclusions:

  • The developed SRS microscopy technique provides a powerful tool for assessing single-cell drug responses.
  • This method offers a faster alternative to traditional drug sensitivity assays.
  • Quantifying 3D cell growth enhances understanding of drug interactions within tumor microenvironments.