Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Combinatorial Gene Control02:33

Combinatorial Gene Control

8.4K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.4K
Master Transcription Regulators02:23

Master Transcription Regulators

6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Generator Voltage Control01:21

Generator Voltage Control

160
Generator voltage control is crucial for maintaining the stable operation of synchronous generators and wind turbines. In older models, a DC generator driven by the rotor delivers DC power to the rotor's field winding, and the power is transferred through slip rings and brushes. In the latest models, static or brushless exciters are used. Static exciters rectify AC power from the generator terminals and then transfer the DC power directly to the rotor. Brushless exciters, on the other hand,...
160
Muscle Stimulation Frequency01:22

Muscle Stimulation Frequency

2.2K
The contraction strength of muscles is regulated by motor neurons, which modulate the frequency of action potentials dispatched to the motor units based on the body's requirements. This process of varying the muscle stimulation frequency allows muscles to contract with a force that is precisely tailored to the needs of the moment, whether lifting a feather or a heavy box.
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
2.2K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

21.1K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
21.1K
RNA Polymerase II Accessory Proteins02:36

RNA Polymerase II Accessory Proteins

9.2K
Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
9.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering commensal microbes for host health.

Cell host & microbe·2026
Same author

Swallowing the Future of Cancer Prevention: Could EsoGuard Revolutionize the Fight Against Esophageal Cancer?

Gastro hep advances·2026
Same author

Daring few, patient many: Division of labor in decentralized foraging collectives.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

De Novo Engineered Living Materials via Elastin-Like Polypeptide-Mediated Self-Assembly.

ACS synthetic biology·2026
Same author

Information-seeking decision strategies mitigate risk in dynamic, uncertain environments.

Journal of the Royal Society, Interface·2026
Same author

Optimizing genetic engineering approaches for protein loading into bacterial extracellular vesicles for vaginal drug delivery.

Journal of controlled release : official journal of the Controlled Release Society·2026

Related Experiment Video

Updated: Jul 9, 2025

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.4K

Tunable Dynamics in a Multistrain Transcriptional Pulse Generator.

David M Zong1, Mehdi Sadeghpour2,3, Sara Molinari2

  • 1Graduate Program in Systems, Synthetic, and Physical Biology, Rice University, Houston, Texas 77005, United States.

ACS Synthetic Biology
|November 28, 2023
PubMed
Summary
This summary is machine-generated.

Synthetic biology circuits in microbial consortia can be tuned by adjusting strain fractions. This method controls gene expression pulses in engineered Escherichia coli communities, simplifying circuit dynamics management.

Keywords:
microbial consortiamulticellular gene circuitsynthetic biology

More Related Videos

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline
10:44

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Published on: December 7, 2021

2.2K
Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

7.7K

Related Experiment Videos

Last Updated: Jul 9, 2025

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells
09:20

Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells

Published on: July 6, 2021

2.4K
Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline
10:44

Inherent Dynamics Visualizer, an Interactive Application for Evaluating and Visualizing Outputs from a Gene Regulatory Network Inference Pipeline

Published on: December 7, 2021

2.2K
Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis
10:25

Real-time Bioluminescence Imaging of Notch Signaling Dynamics during Murine Neurogenesis

Published on: December 12, 2019

7.7K

Area of Science:

  • Synthetic Biology
  • Microbial Consortia
  • Genetic Engineering

Background:

  • Tuning regulatory components in synthetic biology gene circuits is challenging.
  • Synthetic microbial consortia require both intracellular and population-level circuit function.
  • Coordinating gene expression across multiple engineered strains presents unique difficulties.

Purpose of the Study:

  • To demonstrate that synthetic consortium circuit dynamics can be tuned by manipulating strain fractions.
  • To engineer a synthetic microbial consortium exhibiting pulse generation behavior.
  • To develop and validate a mathematical model for predicting consortium dynamics based on strain composition.

Main Methods:

  • Constructed a three-strain synthetic consortium of engineered *Escherichia coli*.
  • Utilized homoserine lactone-mediated intercellular signaling to create a multistrain incoherent type-1 feedforward loop (I1-FFL).
  • Varied the relative population fractions of each engineered strain within the consortium.
  • Developed a mathematical model to simulate and predict the temporal dynamics of the consortium.

Main Results:

  • The engineered consortium functioned as a pulse generator for gene expression.
  • The amplitude of gene expression pulses was effectively tuned by adjusting the relative fractions of the constituent strains.
  • A mathematical model accurately predicted desired pulse characteristics for various population fractions.
  • Experimental results confirmed model predictions, validating the tunability through strain fraction manipulation.

Conclusions:

  • Strain fractions within synthetic microbial consortia are a viable method for tuning circuit dynamics.
  • Intercellular gene circuits in microbial consortia can be effectively controlled by simple adjustments to initial strain proportions.
  • This approach offers a simplified strategy for managing complex behaviors in engineered microbial communities.