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Related Concept Videos

CRISPR01:59

CRISPR

51.7K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Jul 9, 2025

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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A radioactive CRISPR interference system using 89Zr-labeled LbCas12a.

Injoo Hwang1, Jun Young Lee1, Tae-Hyun Kim1

  • 1Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Republic of Korea.

Journal of Controlled Release : Official Journal of the Controlled Release Society
|November 28, 2023
PubMed
Summary
This summary is machine-generated.

Researchers developed isotope-labeled CRISPR proteins for targeted drug delivery and in vivo imaging. This theranostic approach showed therapeutic efficacy in liver fibrosis models, advancing CRISPR applications.

Keywords:
CRISPR interferenceLbCas12aRadiolabeled CRISPRRadiolabeled therapyZirconium-89

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Radiochemistry

Background:

  • CRISPR proteins show promise for drug development but lack suitable delivery systems.
  • Targeted drug delivery requires sensitive and stable agents for in vivo applications.
  • Zirconium-89 (89Zr) offers positron emission for safe, real-time in vivo tracking.

Purpose of the Study:

  • To assess the impact of 89Zr labeling on CRISPR protein functionality.
  • To evaluate the theranostic potential of 89Zr-labeled CRISPR proteins.
  • To demonstrate therapeutic efficacy using CRISPR interference in a liver fibrosis model.

Main Methods:

  • Labeling CRISPR proteins with the positron-emitting isotope 89Zr.
  • In vivo behavioral imaging to confirm protein functionality post-labeling.
  • Therapeutic evaluation in a preclinical mouse model of liver fibrosis.

Main Results:

  • 89Zr labeling did not compromise CRISPR protein functionality during in vivo imaging.
  • Demonstrated therapeutic efficacy of the CRISPR interference system in liver fibrosis.
  • Highlighted the theranostic capabilities of isotope-labeled CRISPR proteins.

Conclusions:

  • 89Zr-labeled CRISPR proteins are functional and suitable for in vivo tracking and therapy.
  • This theranostic approach holds potential for treating liver fibrosis.
  • Findings support the clinical exploration of isotope-labeled CRISPR proteins for in vivo applications.