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Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Published on: July 8, 2012
Murine malaria decreases hemopoietic stem cells.
Blood
|February 1, 1987
Summary
Malaria infection in mice reduces erythropoietic activity and hematopoietic stem cells (CFU-S). Despite adequate erythropoietin (EP) production, stem cell depletion likely contributes to malaria
Area of Science:
- Hematology
- Immunology
- Infectious Diseases
Background:
- Anemia and immunosuppression are key malaria outcomes.
- The precise causes of these outcomes remain unclear.
Purpose of the Study:
- To investigate erythropoietin (EP) production adequacy during malaria.
- To assess the impact on hematopoietic stem cells (CFU-S).
Main Methods:
- Mice infected with Plasmodium species were exposed to simulated high altitude.
- Plasma EP levels were measured via bioassay and RIA.
- Erythropoiesis and CFU-S content were assessed using radioiron incorporation and spleen colony assays.
Main Results:
- Erythropoietic activity decreased in bone marrow and spleen with progressing infection.
- CFU-S content and marrow cellularity declined as parasitemia increased.
- All Plasmodium species stimulated EP production during peak parasitemia.
Conclusions:
- Adequate EP is available during malaria, but erythropoiesis is suppressed.
- Depletion of CFU-S, potentially without compensatory turnover, may drive malaria's pathology.
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