Related Experiment Video

Updated: Jul 9, 2025

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Published on: March 14, 2021

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Key regulator PNPLA8 drives phospholipid reprogramming induced proliferation and migration in triple-negative breast

Zheqiong Tan1,2, Pragney Deme3, Keerti Boyapati1

  • 1Russell H. Morgan Department of Radiology and Radiological Science, Division of Cancer Imaging Research, Johns Hopkins University School of Medicine, Baltimore, MD, USA.

Breast Cancer Research : BCR
|November 29, 2023
PubMed
Abstract

Insights

Researchers identified patatin-like phospholipase domain-containing lipase 8 (PNPLA8) as a key driver in triple-negative breast cancer (TNBC). Targeting PNPLA8 could offer new therapeutic strategies for this aggressive cancer subtype.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Triple-negative breast cancer (TNBC) is an aggressive subtype with poor patient outcomes.
  • Current treatments including surgery and chemotherapy are often insufficient for TNBC.
  • Novel molecular targets are crucial for improving TNBC therapeutic options.

Purpose of the Study:

  • To identify novel therapeutic targets for TNBC.
  • To investigate the role of lipid metabolism in TNBC progression.
  • To combine genomic, functional, and lipidomic analyses.

Main Methods:

  • Utilized a large panel of human breast cancer cell lines and patient samples.
  • Performed comprehensive lipid profiling to analyze metabolic reprogramming.
  • Conducted mechanistic studies to link lipid profiles to cancer cell properties.

Main Results:

  • Phospholipid metabolism is reprogrammed in TNBC cells.
  • Patatin-like phospholipase domain-containing lipase 8 (PNPLA8) is overexpressed in TNBC.
  • PNPLA8 silencing disrupted phospholipid metabolism and affected cell viability, migration, and antioxidation.

Conclusions:

  • PNPLA8 is a key regulator of phospholipid metabolic reprogramming in TNBC.
  • PNPLA8 influences malignant phenotypes including cell viability and migration.
  • PNPLA8 represents a potential novel molecular treatment target for TNBC.
Keywords:
Breast cancerEicosanoidsInvasionMetabolism reprogrammingMigrationPNPLA8PhospholipidProliferationTriple negative

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