Temperature-compensated fiber-optic SPR microfluidic sensor based on micro-nano 3D printing.

Area of Science:

• Optoelectronics
• Biomedical Engineering
• Chemical Sensing

Background:

• Current fiber-optic surface plasmon resonance (SPR) biosensors often lack integrated temperature compensation within microfluidic systems.
• This limitation affects the accuracy and reliability of biosensing, particularly in dynamic environments.

Purpose of the Study:

• To develop a novel temperature-compensated fiber-optic SPR microfluidic sensor using micro-nano 3D printing.
• To enable simultaneous measurement of target biomolecules and ambient temperature for real-time compensation.

Main Methods:

• Fabrication of a fiber-optic SPR microfluidic sensor with two sensing areas on a single fiber using micro-machining.
• Implementation of wavelength division multiplexing for simultaneous signal acquisition from both sensing areas.
• Construction of a temperature compensation matrix for accurate biomolecule quantification.

Main Results:

• The sensor demonstrated a temperature sensitivity of 2.18 nm/°C.
• Sensitivity for berberine detection was 0.2646 nm/(µg/ml) with a limit of detection (LOD) of 0.38 µg/ml.
• The sensor exhibited excellent specificity in detecting berberine within mixed solutions.

Conclusions:

• The developed 3D-printed fiber-optic SPR microfluidic sensor effectively compensates for temperature fluctuations.
• This technology offers a promising platform for accurate and reliable biosensing of specific biomolecules in microfluidic channels.