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Conduction-Dominated Cryomesh for Organism Vitrification.

Zongqi Guo1, Nikolas Zuchowicz1, Jessica Bouwmeester2,3

  • 1Department of Mechanical Engineering, University of Minnesota, Minneapolis, MN, 55455, USA.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|November 29, 2023
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Summary

Improved cryopreservation using conduction-dominated cryomesh enhances cooling rates and viability for biological systems. This advancement supports biodiversity, healthcare, and food production through effective vitrification.

Keywords:
Drosophila embryocoral larvaecryomesh vitrificationcryopreservationheat transferzebrafish embryo

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Area of Science:

  • Cryobiology
  • Materials Science
  • Biotechnology

Background:

  • Vitrification-based cryopreservation is crucial for long-term storage of biological samples.
  • Current cryomesh systems offer rapid cooling but require enhanced rates for broader application.
  • Increased cooling rates are essential for improving biosystem viability and expanding cryopreservation capabilities.

Purpose of the Study:

  • To enhance cryopreservation techniques by improving cooling rates through conductive heat transfer.
  • To investigate the impact of conduction-dominated cryomesh on vitrification efficiency and biosystem survival.
  • To demonstrate the scalability and applicability of the improved cryomesh system for various biological samples.

Main Methods:

  • Development and implementation of a conduction-dominated cryomesh system.
  • Optimization of key parameters: thermal conductivity, mesh dimensions, and liquid nitrogen immersion technique.
  • Testing the system's efficacy on diverse biological models including coral larvae, Drosophila embryos, and zebrafish embryos.

Main Results:

  • Achieved twofold to tenfold increase in cooling rates (0.24 to 1.2 × 10^5 °C min⁻¹).
  • Successfully vitrified coral larvae, Drosophila embryos, and zebrafish embryos with enhanced outcomes.
  • Demonstrated improved viability and successful cryopreservation across µm to mm scale biosystems.

Conclusions:

  • Conduction-dominated cryomesh significantly improves vitrification cooling rates and biosystem viability.
  • The technology provides a scalable solution for biorepositories, agriculture, aquaculture, and scientific research.
  • This advancement broadens the scope of cryopreservation for critical biological resources.