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The pathogenicity and immune effects of different generations of Mycoplasma synoviae on chicken embryos

X Ma1, L Wang1, J Li1

  • 1School of Animal Science and Technology, Ningxia University, Yinchuan, Ningxia, China.

British Poultry Science
|November 29, 2023
PubMed

Insights

Pathogenicity of Mycoplasma synoviae (MS) in chicken embryos decreased with increased passages. Heat shock protein (HSP) and inflammatory gene expression changed, suggesting potential for attenuated vaccine development.

Area of Science:

  • Veterinary immunology
  • Avian pathology
  • Molecular biology

Background:

  • Mycoplasma synoviae (MS) is a key cause of synovitis in poultry.
  • Understanding MS pathogenicity and immune responses is crucial for disease control.

Purpose of the Study:

  • To investigate the pathogenicity and immunological responses of Mycoplasma synoviae (MS) in specific pathogen-free (SPF) chicken embryos across serial passages.
  • To analyze mortality rates and gene expression of heat shock proteins (HSPs) and inflammatory factors.

Main Methods:

  • Serial passages of MS (F1 to F200) in 7-day-old SPF chicken embryos.
  • Mortality rate recording and analysis.
  • Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) for HSPs (HSP27, HSP40, HSP60, HSP70, HSP90) and inflammatory factors (IL-1β, caspase-1, IL-18) in tracheal tissue.

Main Results:

  • Mortality rates decreased significantly with increased MS passages, from 80% in F1 to 10% in F200.
  • Expression of HSP27, IL-1β, HSP40, caspase-1, HSP70, and HSP90 significantly downregulated with increasing passages (P < 0.05).
  • HSP60 expression significantly upregulated with increasing passages (P < 0.05), while IL-18 showed no significant trend.

Conclusions:

  • A clear relationship exists between MS pathogenicity and passage number in chicken embryos.
  • Decreased pathogenicity is associated with altered expression of HSPs and inflammatory genes.
  • Findings provide a basis for selecting MS strains for potential attenuated vaccine development.
Keywords:
Mycoplasma synoviaeSPF chicken embryosdifferent generationsfatalityinflammation

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