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Updated: Jul 9, 2025

Microbial Communities in Nature and Laboratory - Interview
Published on: May 28, 2007
Life, the genome and everything
1Departments of Molecular & Human Genetics, Biochemistry & Molecular Biology, Molecular Virology and Microbiology, and the Dan L Duncan Comprehensive Cancer Center, Baylor College of Medicine, Houston, Texas, USA.
Abstract:
In this issue of the Journal of Bacteriology, N. J. Bonde, E. A. Wood, K. S. Myers, M. Place, J. L. Keck, and M. M. Cox (J Bacteriol 205:e00184-23, 2023, https//doi.org/10.1128/jb.00184-23) used an unbiased transposon-sequencing (Tn-seq) screen to identify proteins required for life when cells lose the RecG branched-DNA helicase (synthetic lethality). The proteins' identities indicate pathways that prevent endogenous DNA damage, pathways that prevent its homology-directed repair (HDR) "strand-exchange" intermediates between sister chromosomes, and pathways that resolve those intermediates. All avoid intermediate pile-up, which blocks chromosome segregation, causing "death-by-recombination." DNA damage is managed to regulate crucial but potentially lethal HDR.
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