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Updated: Jul 9, 2025
siRNA Transfection and EMSA Analyses on Freshly Isolated Human Villous Cytotrophoblasts
Published on: September 20, 2016
Isolation of Human Villous Cytotrophoblastic Cells from Term Placenta for Transfection
Caroline Toudic1, Benoit Barbeau2,3
1Département des sciences biologiques, Centre d'excellence en recherche sur les maladies orphelines-Fondation Courtois, Université du Québec à Montréal, Montreal, QC, Canada.
Abstract:
Isolation of villous cytotrophoblastic cells (vCTBs) from placental tissue provides the possibility of studying differentiation of these cells as well as their function. We describe herein a protocol, in which term placentas are used to isolate a population of vCTB in high quantities. These cells are subsequently used to transfect siRNA through microporation and thereby to achieve efficient silencing of targeted genes.
