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Ex Vivo Perfusion of the Rodent Placenta

Ex Vivo Perfusion of the Rodent Placenta

Published on: May 30, 2019

Precision-Cut Slice Culture Method for Rat Placenta.

Fusun Gundogan^1,2, Jeffrey Gilligan³, Suzanne de la Monte^4,5,6

¹Regional Perinatal Pathology, Kaiser Permanente Oakland Medical Center, Oakland, CA, USA. fusun.x.gundogan@kp.org.

Methods in Molecular Biology (Clifton, N.J.)

|November 29, 2023

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Organotypic slice cultures retain complex tissue characteristics, offering advantages over traditional cell models. This study details a method for creating rat placenta organotypic slice cultures for research applications.

Area of Science:

* Reproductive biology
* Developmental toxicology
* Tissue engineering

Background:

* Traditional cell culture models (monolayer) often fail to replicate complex in vivo tissue environments.
* Organotypic slice cultures preserve three-dimensional (3D) tissue architecture, multi-lineage cell populations, and intricate functional attributes.
* These cultures serve as valuable models for studying tissue responses to various agents and predicting in vivo outcomes.

Purpose of the Study:

* To present a standardized methodology for the preparation of organotypic slice cultures derived from rat placenta.
* To highlight the utility of these cultures as a research model in reproductive and toxicological studies.

Main Methods:

* Detailed protocol for dissecting and sectioning rat placenta tissue.

Keywords:

Methodology Organotypic Placenta Slice culture Trophoblast

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Last Updated: Jul 9, 2025

Ex Vivo Perfusion of the Rodent Placenta

Ex Vivo Perfusion of the Rodent Placenta

Published on: May 30, 2019

Organotypic Slice Culture of E18 Rat Brains

Organotypic Slice Culture of E18 Rat Brains

Published on: July 11, 2007

Preparation of Rat Brain Aggregate Cultures for Neuron and Glia Development Studies

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* Establishment of conditions for maintaining the viability and structural integrity of placental organotypic slices.

* Preparation of slices for subsequent exposure and analysis.

Main Results:

* Successful generation of viable organotypic slice cultures from rat placenta.
* Demonstrated retention of key placental structural and cellular features in the prepared slices.
* Established a foundation for utilizing these cultures in experimental investigations.

Conclusions:

* Organotypic slice cultures of rat placenta are a feasible and advantageous model system.
* This method facilitates the study of placental biology and responses to xenobiotics.
* These cultures offer a more predictive model compared to traditional cell culture techniques.