Related Experiment Video
Updated: Jul 9, 2025
Ex Vivo Perfusion of the Rodent Placenta
Published on: May 30, 2019
Precision-Cut Slice Culture Method for Rat Placenta
Fusun Gundogan1,2, Jeffrey Gilligan3, Suzanne de la Monte4,5,6
1Regional Perinatal Pathology, Kaiser Permanente Oakland Medical Center, Oakland, CA, USA. fusun.x.gundogan@kp.org.
Abstract:
Organotypic slice cultures provide several advantages over primary and cell line culture models. Unlike monolayer cultures, organotypic slice cultures retain the tissue's 3-D architecture, multi-lineage cell populations, and many complex functional characteristics. Organotypic slice cultures can be exposed to biological, pharmacological, or environmental agents and analyzed to predict in vivo responses. Herein, we describe a method for preparing organotypic slice cultures generated from rat placenta.
